Recommended Working Volumes
Single-well, 24-well and 96-well inserts are available to meet a range of application needs. The protocols printed in this section of the guide can be adjusted to accommodate a range of platforms. Please use the following table as a reference for scaling liquid additions in Millicell protocols. | Well Diameter (mm) | Membrane Surface Area (cm2) | Apical Volume (ml) | Basolateral Volume (ml) (24- or 96-well Receiver Tray) | Basolateral Volume (ml) (single-well Receiver Tray) |
| 6-well standing inserts | 30 | 4.2 | 1.5 | 2.0 | n/a |
| 6-well hanging inserts | 27 | 4.5 | 2.0 | 4.2* | n/a |
| 12-well hanging inserts | 15 | 1.1 | .4 | 2.0* | n/a |
| 24-well standing inserts | 12 | .6 | .4 | .6 | n/a |
| 24-well hanging inserts | 9 | .3 | .2 | 1.3* | n/a |
| Millicell-24 Cell Culture Insert Plate | 11 | .7 | .3 | .6 | 28 |
| Millicell-96 Cell Culture Insert Plate | 5 | .1 | .075 | .25 | 32 |
| MultiScreen-MIC Filter Plate | 7 | .3 | .075 | .15 | n/a |
* The basolateral volumes are measured in standard plastic culture plates with 18 mm well heights and are at equal head height with the apical well.
