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Millipore Applications Bibliography |
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| Title: | Preparing Circular DNA for AFM Imaging | |
| Author/s: | Wang H, Li Z, Wang E | |
| Reference: | American Bitoechnology Laboratory, August 2000:38 | |
| Description: | Application Note | |
| Water System: | Milli-Q Ultrapure Water Systems | |
Summary/Comments: The Atomic Force Microscope (AFM) has become a powerful tool for investigating biomolecules since its invention in 1986. DNA, which plays an important role in molecular biology, is under intensive study using AFM. The investigation includes DNA morphology and DNA interaction with other molecules, which broadens our understanding of life processes and mechanisms.1,2 Immobilizing DNA is key to imaging it by AFM. It is difficult to obtain circular DNA because plasmid DNA exists in a surperhelical form under normal conditions. Generally, the methods of heating DNA solution and topoisomerase are used to obtain circular DNA.3 However, heating in water makes it easy to denature DNA, and using topoisomerase to obtain circular DNA is costly and time consuming. For this study, Co(phen)33 + was selected as an intermediate to immobilize plasmid. A Milli-RO 10 and Milli-Q Plus system were used in the protocol. Click on the PDF above to view the entire document. | ||