The surface of derivatized CPG provides reactive functional groups for covalent attachment of ligands. In addition, derivatized CPG is the solid support of choice for solid phase DNA synthesis. The media’s well defined particle size, pore size and pore volume, and surface area are critical to achieve high efficiency, purity and reproducibility of synthesis. Millipore offers derivatized CPG in a variety of chemistries and pore sizes.
Technical Publications
- DNA Nucleoside Controlled Pore Glass (CPG®) Media: Solid support for DNA synthesis
The ability for research laboratories to cost-effectively synthesize DNA and RNA molecules in-house has greatly improved over the course of the past two decades. Automated oligonucleotide synthesizers are now available in most laboratories. Readily accessible technology has allowed synthetic molecules to be pervasively used in sequencing and in polymerase chain reaction (PCR) amplification techniques. Phosphoramidite chemistry is generally used for the synthesis of the oligonucleotides, which is carried out by sequentially adding the desired nucleotide units to an immobilized oligonucleotide chain.
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- Derivatized Controlled Pore Glass (CPG®) Media: Solid supports for covalent attachment of biological molecules
Millipore’s derivatized CPG media provide reactive functional groups for covalent attachment of ligands. The surface of Millipore’s CPG particles, a porous borosilicate material, is modified by reacting silanol groups on the glass surface with suitable, functionalized silanes. Using a proprietary chemistry, the organic groups are bonded then to the glass surface through stable and consistent covalent silicon-oxygen-silicon bonds.
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