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MILLIPLEX MAP EpiQuant Products

  
The MILLIPLEX MAP EpiQuant platform unites two powerful technologies –
EpiQuant technology with the Luminex xMAP platform –
to bring you the most advanced in quantitative cell signaling.
Answering the challenges of multiplex cell signaling,
MILLIPLEX MAP EpiQuant allows you to:
  • Design small- or large-plexed panels easily

  • Customize your assay by selecting analytes you want to study

  • Obtain quantitative results at picomolar sensitivities

  • Measure phosphorylation concentrations of multiple sites on the same protein – all in one well

  • Measure both total and phosphoprotein – all in the same well

  • Utilize all the advantages and features associated with the MILLIPLEX MAP assay format

Learn more...

      
MILLIPLEX MAP EpiQuant Sample Preparation Kit (Catalogue Number: MPEQ-SP)*
A sample prep kit containing all the reagents you need to prepare up to 5 mL of sample lysates, including:
  • Cell lysis
  • Protein denaturing
  • Protein fragmentation

*Kit required to run MILLIPLEX MAP EpiQuant multiplex assays
  

      
MILLIPLEX MAP EpiQuant Phosphotyrosine Cell Signaling Panel 1 (Catalogue Number: MPEQ-100K)
  • All the components and reagents you need to simultaneously detect multiple phosphotyrosine sites in a small sample size
  • The ability to choose analytes from different pathways to design a custom kit
  • Standards included to obtain quantitative picomolar-range results
  • Quality controls provided to qualify assay performance
  • Analytically validated panels that yield consistent analyte profiles irrespective of plex size
  • Standardized standard curve ranges across analytes and lots to ensure lot-to-lot consistency
  • Panels that meet stringent manufacturing criteria to ensure batch-to-batch reproducibility
  

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Analytes in MILLIPLEX MAP EpiQuant Phosphotyrosine Cell Signaling Panel 1
Cbl (Tyr700/731/774)
Crk (Tyr221/239)
CUB domain containing protein 1 (Tyr734)
EGFR (Tyr1110/1125)
Ezrin (Tyr477)
FAK (Tyr397/407
FAK (Tyr861)
FRS2 (Tyr436)
Fyb (Tyr97/651)
Gab1 (Tyr285/307/317)
Gab2 (Tyr266)
Gab2 (Tyr584)
Growth hormone receptor (Tyr566)
HER2 (Tyr1023)
HGFR (Tyr1003)
IL-15Rα chain (Tyr227)
IRS1 (Tyr612)
ITK (Tyr180)
JAK3 (Tyr785)
JNK 1/2/3 (Tyr185/185/223)		LAT (Tyr255)
p38/MAPKα (Tyr181)
PDK1 (Tyr373/376)
PI3K p110γ (Tyr485)
Plakoglobin/g-catenin (Tyr548)
PLCγ1 (Tyr1253)
PLCγ2 (Tyr1197/1217)
Raf-1 (Tyr340/341)
Rap GEF1/GRF2 (Tyr504)
SCF38 (Tyr20)
SH2 domain containing protein 3A (Tyr231)
SH2 domain containing protein 3A (Tyr95)
Shc (Tyr349/350)
SPTAN1 (Tyr1176)
STAT3 (Tyr705)
Transferrin Receptor (Tyr20)
Tyrosine protein kinase Tec (Tyr206)
VEGFR1 (Tyr1169)
WASP (Tyr291)
ZAP70 (Tyr126)

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Assay Characteristics



MILLPLEX MAP EpiQuant Peptide Standard Curves

Dilution series of standard peptides from 0.6 pM to 1 nM shows Median Fluorescence Intensity remains linear over 2-3 orders of magnitude, for quantitative power.
Click image to enlarge

Dynamic Range:
  • 0.32 pM – 1,000 pM
Specificity
  • There is no significant cross-reactivity observed between the antibodies and/or non-specific analytes in this panel.
Precision:
  • Intra-Assay: 1.8% – 17.5%
  • Inter-Assay: 5.6% – 19.6%
Accuracy:
  • 81.2% – 119.6%
Sensitivity:

MinDC < 0.32 pM
MinDC = 0.33 - 1.10 pM
MinDC = 1.11 - 3.0 pM
MinDC > 3.1 pM
Cbl (pY700/731/774)Crk (pY221/239)CUB domain containing protein 1 (pY734)Fyb (pY697/651 (iso1/2))
EGFR (pY1110/1125)FAK (pY861)FRS2 (pY436)LAT (pY255)
Ezrin (pY477)Gab2 (pY266)Gab1 (pY285/307/317)Plakoglobin (γ catenin) (pY548)
FAK (pY397/407)Gab2 (pY584)HER2 (pY1023)SCF38 (pY20)
JNK1/2/3 (pY185/185/223)Growth hormone receptor (pY566)IL-15 receptor α chain (pY227)SH2 domain containing protein 3A (pY95)
p38MAPKα (pY181)HGFR (met) (pY1003)JAK3 (pY785)Tec (pY206)
PDK1 (pY373/376)IRS1 (pY612)RapGEF1 (GRF2) (pY504 (522 in iso1))VEGFR1 (pY1169)
Pl3Kp110δ (pY485)ITK (pY180)TfR (pY20)WASP (pY291)
PLCg1 (pY1253)Shc (pY349/350)
PLCg2 (pY1197/1217)SPTAN1 (pY1176)
Raf 1 (pY340/341)STAT3 (pY705)
SH2 domain containing protein 3A (pY231)ZAP70 (pY126)



Learn more about about MILLIPLEX MAP EpiQuant or to receive a quote,
e-mail drugdiscovery@millipore.com and a Millipore account specialist will contact you.


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