MILLIPLEX MAP Comparative Data

The performance characteristics of MILLIPLEX MAP Cell Signaling MAPmates were benchmarked against analytes selected from two competitors. A total of five phosphoprotein analytes (see table below) were selected based on availability from Millipore and competitor companies, as well as maximal signaling pathway overlap.

Target	Lysate Tested
pEGFR	A431/EGF
pJNK/SAPK1	HeLa/HS-Ars
pERK1/2	A431/EGF
p-p38	HeLa/HS-Ars
p-p70 S6K	A431/EGF

Manufacturer instructions for cell lysis and sample preparation as well as protocol assay procedures were followed rigorously for each of the cell signaling kits. The results below with the fold induction values demonstrate the superior product performance of the MILLIPLEX MAP Cell Signaling product line.

Advantages of MILLIPLEX MAP Cell Signaling Portfolio –
Simpler, Easier Multiplexing

Protocol & Assay Design: Very straightforward and user-friendly
	• No additional sample treatment for some MAPmates
	• One protocol design for all MAPmates
Kit Format: All kits designed with the same format for multiplexing with the least optimization
Buffers Required: Only two buffers required – Dilution and Wash buffers Incubation Time & Temperature: Overnight incubation at 4° C
	• Shorter incubation time at room temperature possible
Standards & Controls:
	• Lyophilized negative control (unstimulated lysate) included in MILLIPLEX MAP Buffer & Detection Kit
	• Lyophilized positive control (stimulated lysate) included with MAPmates