GPCR Cell Lines

ChemiScreen Ca²⁺-Optimized GPCR Stable Cell Lines

Our Stable Calcium-Optimized GPCR Cell lines combine two proprietary technologies (expression vector and ChemiScreen cells) to improve the functional expression of GPCRs.

To meet the demands of HTS and selectivity profiling, our ChemiScreen GPCR cell lines are uniquely adapted for calcium-based functional screening. Endogenous expression of promiscuous G proteins in ChemiScreen cells funnel GPCR signaling to a common calcium readout, regardless of G protein coupling status. The result is a robust and uniform platform for cell-based screening and profiling.

For recent additions, see our brochure:
GPCR Target Panel Q1 2009 Update

» View Product Listing below

Advantages of Millipore GPCR cell lines

High functional expression of receptors at the cell surface
Stable cell lines for increased reliability and reproducibility
Single and simple functional readout
Signaling to Ca2+, regardless of G protein coupling status
Identical assay conditions and data analysis for straightforward comparative studies

Proprietary pHS expression vector and ChemiScreen cell lines enhance GPCR cell surface expression:

Millipore’s stable ChemiScreen Optimized GPCR cell lines provide a standard assay setup and common readout for all GPCR targets

Product Listing - Stable Calcium-Optimized GPCR Cell lines

Class	Ligand Type	GPCR Family	GPCR Target (Human unless noted)	Cell Line Host	ChemiScreen Ca2+-Optimized Cell Line Cat. No.	*
A	non-peptide	Acetylcholine (muscarinic)	M1	Chem-1	HTS044C
A	non-peptide	Acetylcholine (muscarinic)	M2	Chem-1	HTS115C
A	non-peptide	Acetylcholine (muscarinic)	M3	Chem-1	HTS116C
A	non-peptide	Acetylcholine (muscarinic)	M4	Chem-4	HTS117C
A	non-peptide	Acetylcholine (muscarinic)	M5	Chem-1	HTS075C
A	non-peptide	Adenosine	A1	Chem-3	HTS047C
A	non-peptide	Adenosine	A2B	Chem-3	HTS051C
A	non-peptide	Adenosine	A3	Chem-3	HTS052C
A	non-peptide	Adrenergic	alpha1A	Chem-1	HTS087C
A	non-peptide	Adrenergic	alpha1B	Chem-1	HTS158C
A	non-peptide	Adrenergic	alpha1D	Chem-1	HTS216C
A	non-peptide	Adrenergic	alpha2A	Chem-1	HTS096C
A	non-peptide	Adrenergic	beta 1	Chem-1	HTS104C
A	non-peptide	Adrenergic	beta 2	Chem-1	HTS073C
A	non-peptide	Adrenergic	beta 3	Chem-10	HTS159C
A	non-peptide	alpha-Ketoglutarate	OXGR1 , GPR99 , GPR80	Chem-1	HTS191C
A	peptide	Anaphylotoxin	C3aR	Chem-1	HTS016C
A	peptide	Anaphylotoxin	C5aR	Chem-1	HTS017C
A	peptide	Angiotensin	AT1	Chem-1	HTS064C
A	peptide	Apelin	APJ	Chem-5	HTS068C
A	peptide	Bombesin	BB1	Chem-1	HTS123C
A	peptide	Bombesin	BB2	Chem-1	HTS084C
A	peptide	Bombesin	BB3	Chem-1	HTS160C
A	peptide	Bradykinin	B2	Chem-1	HTS041C
B	peptide	Calcitonin	CGRP1	Chem-1	HTS172C
C	non-peptide	Calcium sensor	CaS	Chem-1	HTS137C
A	non-peptide	Cannabinoid	CB1	Chem-1	HTS019C
A	non-peptide	Cannabinoid	CB2	Chem-4	HTS020C
A	peptide	Chemoattractant	ChemR23 / CMKLR1	Chem-1	HTS071C
A	peptide	Chemokine	CCR1	Chem-1	HTS005C
A	peptide	Chemokine	CCR10	Chem-1	HTS014C
A	peptide	Chemokine	CCR2B	Chem-1	HTS007C
A	peptide	Chemokine	CCR3	Chem-1	HTS008C
A	peptide	Chemokine	CCR4	Chem-5	HTS009C
A	peptide	Chemokine	CCR5, rhesus macaque	Chem-1	HTS010C
A	peptide	Chemokine	CCR6	Chem-1	HTS011C
A	peptide	Chemokine	CCR7	Chem-1	HTS012C
A	peptide	Chemokine	CCR8	Chem-1	HTS013C
A	peptide	Chemokine	CCR9	Chem-1	HTS036C
A	peptide	Chemokine	CX3CR1	Chem-4	HTS015C
A	peptide	Chemokine	CXCR1 / IL-8a	Chem-1	HTS001C
A	peptide	Chemokine	CXCR2 / IL-8b	Chem-1	HTS002C
A	peptide	Chemokine	CXCR3	Chem-1	HTS003C
A	peptide	Chemokine	CXCR4	Chem-1	HTS004C
A	peptide	Chemokine	CXCR5	Chem-10	HTS055C	NEW
A	peptide	Chemokine	CXCR6	Chem-5	HTS054C
A	peptide	Chemokine	XCR1 / GPR5	Chem-4	HTS053C
A	peptide	Cholecystokinin	CCK1 / CCKa	Chem-1	HTS184C
A	peptide	Cholecystokinin	CCK2 / CCKb	Chem-1	HTS083C
B	peptide	CRF Receptor	CRF1	Chem-1	HTS023C
B	peptide	CRF Receptor	CRF2	Chem-1	HTS024C
A	non-peptide	Dopamine	D1	Chem-6	HTS102C
A	non-peptide	Dopamine	D2L	Chem-7	HTS039C
A	non-peptide	Dopamine	D2L	CHO	HTS039C2	NEW
A	non-peptide	Dopamine	D4	Chem-5	HTS223C	NEW
A	non-peptide	Dopamine	D5	Chem-1	HTS129C
A	peptide	Endothelin	ETA	Chem-1	HTS070C
A	peptide	Endothelin	ETB	CHO	HTS046C
A	non-peptide	Free Fatty Acid	FFA3 / GPR41	Chem-4	HTS135C
A	non-peptide	Free Fatty Acid	FFA2 / GPR43	Chem-1	HTS063C
C	non-peptide	GABAB	GABAB1b	Chem-1	HTS119C
A	peptide	Galanin	GAL1	Chem-1	HTS094C
A	peptide	Galanin	GAL2	Chem-1	HTS186C
A	peptide	Ghrelin	Ghrelin / GHSR-1a / growth hormone secretagogue receptor	Chem-1	HTS187C
B	peptide	Glucagon	GIP	Chem-9	HTS134C
B	peptide	Glucagon	GLP-1	Chem-9	HTS163C
B	peptide	Glucagon	GLP-1	CHO	HTS163C2	NEW
B	peptide	Glucagon	GLP-2	Chem-11	HTS164C	NEW
B	peptide	Glucagon	glucagon / GCG	Chem-1	HTS112C
B	peptide	Glucagon	secretin receptor / SEC	Chem-1	HTS174C
C	non-peptide	Glutamate (metabotropic)	mGlu1	Chem-3	HTS145C	NEW
C	non-peptide	Glutamate (metabotropic)	mGlu2	Chem-1	HTS146C
A	peptide	Glycoprotein hormone	TSH / TSHR	Chem-10	HTS133C
A	peptide	GnRH	GnRH / LHRH	Chem-1	HTS027C
A	non-peptide	Histamine	H1	Chem-3	HTS050C
A	non-peptide	Histamine	H2	Chem-2	HTS086C
A	non-peptide	Histamine	H3	Chem-3	HTS029C
A	peptide	KiSS1-derived peptide	KiSS1 / GPR54	Chem-1	HTS032C
A	non-peptide	Leukotriene	BLT1	Chem-1	HTS042C
A	non-peptide	Leukotriene	CysLT1	Chem-1	HTS061C
A	non-peptide	Leukotriene	CysLT2	Chem-1	HTS088C
A	non-peptide	Lysophospholipid	LPA1 / EDG2	Chem-1	HTS089C
A	non-peptide	Lysophospholipid	LPA3 / EDG7	Chem-1	HTS130C
A	non-peptide	Lysophospholipid	GPR92 / LPA5	Chem-1	HTS209C
A	non-peptide	Lysophospholipid	S1P1 / EDG1	Chem-4	HTS176C
A	non-peptide	Lysophospholipid	S1P2 / EDG5	Chem-1	HTS078C
A	non-peptide	Lysophospholipid	S1P3 / EDG3	Chem-1	HTS097C
A	non-peptide	Lysophospholipid	S1P4 / EDG6	Chem-5	HTS192C
A	non-peptide	Lysophospholipid	S1P5 / EDG8	Chem-5	HTS193C
A	peptide	Mas-related gene	MRGX1 / MRGPRX1	Chem-1	HTS122C
A	peptide	Mas-related gene	MRGX2 / MRGPRX2	Chem-1	HTS058C
A	peptide	Mas-related gene	MRGPRD / MrgD	Chem-1	HTS206C
A	peptide	Melanin-concentrating hormone	MCHR1 / GPR24	Chem-1	HTS025C
A	peptide	Melanin-concentrating hormone	MCHR2	Chem-1	HTS026C
A	peptide	Melanocortin	MC2	Chem-1	HTS021C
A	peptide	Melanocortin	MC4	Chem-3	HTS105C
A	peptide	Melanocortin	MC5	Chem-10	HTS155C
A	peptide	Motilin	motilin receptor / MTLR / GPR38	Chem-1	HTS121C
A	peptide	Neuromedin U	NMU1	Chem-1	HTS062C
A	peptide	Neuromedin U	NMU2	Chem-1	HTS098C
A	peptide	Neuropeptide B/W	NPBW1 / GPR7	Chem-4	HTS180C
A	peptide	Neuropeptide Y	Y2	Chem-1	HTS066C
A	peptide	Neuropeptide Y	Y4	Chem-1	HTS204C
A	peptide	Neurotensin	NTR1 / NTS1	Chem-1	HTS034C
A	peptide	N-formylpeptide	FPR1	Chem-1	HTS018C
A	peptide	N-formylpeptide	FPRL1 / AXL / HM63	Chem-1	HTS056C
A	non-peptide	Nicotinic Acid	GPR109A	Chem-4	HTS201C
A	peptide	Opioid	Delta / OP1 / DOP / DOR	Chem-1	HTS100C
A	peptide	Opioid	Kappa / OP2 / KOP / KOR	Chem-1	HTS095C
A	peptide	Opioid	Mu / OP3 / MOP / MOR	Chem-5	HTS101C
A	peptide	Opioid	NOP / ORL1 / OP4	Chem-1	HTS040C
A	peptide	Orexin	OX1	Chem-1	HTS175C
A	peptide	Orexin	OX2	Chem-1	HTS045C
A	peptide	Oxytocin	OT	Chem-1	HTS090C
A	non-peptide	P2 / Purinergic (metabotropic)	P2Y1	1321N1	HTS049C
A	non-peptide	P2 / Purinergic (metabotropic)	P2Y2	1321N1	HTS210C
A	non-peptide	P2 / Purinergic (metabotropic)	P2Y4	1321N1	HTS211C
A	non-peptide	P2 / Purinergic (metabotropic)	P2Y11	1321N1	HTS213C
A	non-peptide	P2Y / Purinergic (metabotropic)	P2Y12	U2OS	HTS195C	NEW
A	peptide	Peptide P518	GPR103 / QRFP	Chem-1	HTS189C
A	non-peptide	Platelet Activating Factor	PAF	Chem-1	HTS076C
A	peptide	Prokineticin	PK1 / PKR1	Chem-1	HTS074C
A	peptide	Prokineticin	PK2 / PKR2	Chem-1	HTS182C
A	peptide	Prolactin-releasing peptide	PRP / PrRP / GPR10	Chem-1	HTS057C
A	non-peptide	Prostanoid	DP	Chem-1	HTS091C
A	non-peptide	Prostanoid	EP1	Chem-1	HTS099C
A	non-peptide	Prostanoid	EP2	Chem-9	HTS185C
A	non-peptide	Prostanoid	EP3	Chem-1	HTS092C
A	non-peptide	Prostanoid	EP4	Chem-1	HTS142C
A	non-peptide	Prostanoid	FP	Chem-1	HTS093C
A	non-peptide	Prostanoid	IP1 / PGI2	Chem-1	HTS131C
A	non-peptide	Prostanoid	TP / TXA2 / PGH2	Chem-1	HTS081C
B	peptide	PTH receptor	PTH1	Chem-1	HTS030C
B	peptide	PTH receptor	PTH2	Chem-1	HTS173C
A	non-peptide	Serotonin	5-HT1A	Chem-3	HTS107C
A	non-peptide	Serotonin	5-HT2A	Chem-1	HTS082C
A	non-peptide	Serotonin	5-HT2B	CHO	HTS109C
A	non-peptide	Serotonin	5-HT2C	Chem-1	HTS132C
A	non-peptide	Serotonin	5-HT4	Chem-1	HTS110C	NEW
A	non-peptide	Serotonin	5-HT6	Chem-10	HTS111C	NEW
A	peptide	Somatostatin	sst2	Chem-1	HTS028C
A	peptide	Somatostatin	sst3	Chem-1	HTS171C
A	peptide	Somatostatin	sst4	Chem-1	HTS125C
A	peptide	Somatostatin	sst5	Chem-1	HTS139C
A	non-peptide	SPC/LPC /proton-sensor	GPR68/OGR1	Chem-1	HTS153C
A	non-peptide	Succinate	SUCNR1/GPR91	Chem-1	HTS241C	NEW
A	peptide	Tackykinin /neurokinin	NK1	Chem-1	HTS080C
A	peptide	Tackykinin /neurokinin	NK2	Chem-1	HTS144C
A	peptide	Tackykinin /neurokinin	NK3	Chem-1	HTS190C
A	peptide	TRH	TRH	Chem-1	HTS126C
A	peptide	Urotensin II	UT / UT1 / GPR14	Chem-2	HTS033C
A	peptide	Vasopressin	V1A	Chem-1	HTS059C
A	peptide	Vasopressin	V1A, rat	Chem-1	HTS177C
A	peptide	Vasopressin	V1B	Chem-1	HTS136C
A	peptide	Vasopressin	V2	Chem-1	HTS060C
B	peptide	VIP/PACAP	PAC1 long isoform / PACAP	Chem-1	HTS114C
B	peptide	VIP/PACAP	VPAC1 / VIP1	Chem-1	HTS043C
B	peptide	VIP/PACAP	VPAC2 / VIP2	Chem-1	HTS079C
A	non-peptide	Orphan	GPR39	HEK293	HTS217C	NEW
		Control	Chem-1	CHEM-1 PARENTAL CELL LINE	HTSCHEM-1
		Control	Chem-2	CHEM-2 PARENTAL CELL LINE	HTSCHEM-2
		Control	Chem-3	CHEM-3 PARENTAL CELL LINE	HTSCHEM-3
		Control	Chem-4	CHEM-4 PARENTAL CELL LINE	HTSCHEM-4	NEW
		Control	Chem-5	CHEM-5 PARENTAL CELL LINE	HTSCHEM-5	NEW
		Control	Chem-9	CHEM-9 PARENTAL CELL LINE	HTSCHEM-9	NEW
		Control	Chem-10	CHEM-10 PARENTAL CELL LINE	HTSCHEM-10	NEW