Quantitative Cell Imaging Kits

Whether images are being capture with a manual fluorescence microscope or a state of the art automated high content analysis (HCA) instrument, a high quality image is required for reliable and quantitative data. This is what Millipore’s QCI kits deliver and much more. Moreover, the daunting task of assay develop is a thing of the past as Millipore’s QCI kits are designed to use out of the box.

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Cell imaging can reveal effects that would be missed by other assay types. The neurotoxic effects of nocodazole treatment on neurite length can be seen in the images (above: DMSO control, left; 0.1 μM, middle; and 10 μM, right) and quantified (below: red line). Whereas, nacodazole had no effects using a MTT assay (below: blue line) a common viability assays that measures the metabolic activity of mitochondrial reductase.

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The Erk phosphorylation response of Chem-1 cells (wildtype – above, left images; OX1 receptor transfected - above, right images) upon treatment with vehicle (top images) or 10 μM Orexin A (bottom images) can be quantified to produce dose response curves (below) using Millipore’s Phospho–ERK1/2 (Thr185/Tyr187) Assay Kit (catalogue number HCS230) .

Millipore’s QCI kits are easy to use and include the following components:

• 5-96 well assay plates
• Wash buffer
• Fixation buffer
• Permeablization buffer
• Stains and Antibodies supplied in an easy-to-dilute stock solution
• Reference compounds supplied in easy-to-dilute stock solution
• Handbook with detailed protocols and sample data

Millipore’s QCI kits cover a diverse range of biology and applications
View Product Listing:

• Neurite Outgrowth
• Cell Cycle & Proliferation
• DNA Damage & Apoptosis
• Cellular Stress & Toxicity
• Cellular Signaling
• Staining & Buffer Toolkits

Watch an instructional video on performing Quantitative Cell Imaging using Millipore’s Neuronal and Astrocyte Co-Culture Assay Kit (catalogue number HCS226) as an example:

• Journal of Visualized Experiments (JoVE) video

Related Information:

Brochures for QCI / HCA:

• Product listing
• Neurite Outgrowth
• Cell Cycle & Proliferation
• DNA Damage & Apoptosis
• Cell Stress
• Neurotoxicity

Posters for QCI / HCA:

• Novel assays for neurotoxicity, neural development and neuronal/glia interactions via multiplexed HCA of neurons and astrocytes
• High Content Analysis of betaIII-tubulin and synaptophysin enables sensitive detection of neuronal differentiation and neurotoxicity in a variety of cellular models
• High Content Analysis assays for cell cycle analysis, cytotoxicity profiling and drug screening
• Multiplexed High Content Analysis for the investigation of early-stage oxidative stress, DNA damage and apoptosis
• Development of assays for High Content Analysis of DNA damage and apoptosis
• High Content Analysis assays for signaling pathways and phosphorylation events
• Quantitation of Erk activation via automated cellular imaging and analysis
• Development of an assay to quantitate beta-catenin expression and distribution via automated cellular imaging and analysis

Neurite Outgrowth

Catalogue Number	Application	Description
HCS220	Neurite Outgrowth Assay Kit (βIII-Tubulin)	Neuronal Development, Neurotoxicity
HCS221	Gliosis Assay Kit (GFAP)	Gliosis, Neurotoxicity, Astrocyte Differentiation
HCS222	Neuronal and Astrocyte Co-Culture Assay Kit	Neurotoxicity, Gliosis, Neuronal and Astrocyte Differentiation
HCS226	Neurite Outgrowth and Synaptic Activity Assay Kit	Neurotoxicity, Neuronal Development, Synaptogenesis

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Cell Cycle & Proliferation

Catalogue Number	Application	Description
HCS201	BrdU Assay Kit	Cell Proliferation, Cell Cycle Analysis, Cytotoxicity
HCS202	Cyclin B1 Assay Kit	Cell Cycle Analysis
HCS203	Phospho-Histone H3(Ser 10) Assay Kit (Mouse antibody)	Mitosis, Cell Cycle, Aurora Kinase Profiling, Epigenetics
HCS204	Phospho-Histone H3(Ser 10) Assay Kit (Rabbit antibody)	Mitosis, Cell Cycle, Aurora Kinase Profiling, Epigenetics
HCS205	Ki-67 Assay Kit (Rabbit antibody)	Cell Proliferation, Cytotoxicity
HCS206	Ki-67 Assay Kit (Mouse antibody)	Cell Proliferation, Cytotoxicity
HCS209	Phospho-Histone H3(Ser 10) and Ki-67 Assay Kit	Cell Proliferation, Mitotic Index, Cell Cycle, Epigenetics
HCS210	Cyclin B1 and Ki-67 Assay Kit	Cell Proliferation, Cell Cycle
HCS211	Phospho-Histone H3(Ser 10) and Cyclin B1 Assay Kit	Mitosis, Cell Cycle Analysis, Epigenetics
HCS212	BrdU and Phospho-Histone H3(Ser 10) Assay Kit	Cell Proliferation, Cell Cycle, Mitotic Index, Epigenetics, Cytotoxicity
HCS213	BrdU and Ki-67 Assay Kit	Cell Proliferation, Cell Cycle Analysis, Cytotoxicity
HCS216	Phospho-Histone H3(Ser 10) and α-Tubulin Assay Kit	Cell Proliferation, Microtubule Dynamics, Cancer, Epigenetics

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DNA Damage & Apoptosis

Catalogue Number	Application	Description
HCS223	p53 Assay Kit	DNA Damage and Repair
HCS224	Phospho-Histone H2A.X(Ser 139)	Genotoxicity, Apoptosis, Cellular Stress, Epigenetics
HCS225	Phospho-Histone H2A.X(Ser 139) and p53 Assay Kit	Genotoxicity, DNA Damage, Apoptosis, Epigenetics
HCS234	p21 Assay Kit	Cyclin-Dependent Kinase, Inhibition, DNA Damage, Cellular Stress
HCS235	p53 and p21 Assay Kit	Cell Cycle, DNA Damage, Cellular Stress, Pathway Profiling
HCS236	Cytochrome C Assay Kit	Apoptosis, Mitochondrial Health

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Cellular Stress & Toxicity

Catalogue Number	Application	Description
HCS100	Hepatotoxicity Assay Kit	Hepatotoxicity, Multiparameter Cytotoxicity Analysis
HCS213	BrdU and Ki-67 Assay Kit	Cell Proliferation, Cell Cycle Analysis, Cytotoxicity
HCS221	Gliosis Assay Kit (GFAP)	Gliosis, Neurotoxicity, Astrocyte Differentiation
HCS225	Phospho-Histone H2A.X(Ser 139) and p53 Assay Kit	Genotoxicity, DNA Damage, Apoptosis, Epigenetics
HCS226	Neurite Outgrowth and Synaptic Activity Assay Kit	Neurotoxicity, Neuronal Development, Synaptogenesis
HCS231	p38 MAPK Assay Kit	Cell-based Kinase Screening, Cell Signaling, Cellular Stress
HCS232	Manganese Superoxide Dismutase (MnSOD) Assay Kit	Oxidative Stress, Mitochondrial Health
HCS233	MnSOD and Phospho-Histone H2A.X(Ser 139) Assay Kit	Oxidative Stress, Genotoxicity, Epigenetics, Apoptosis
HCS235	p53 abd p21 Assay Kit	Cell Cycle, DNA Damage, Cellular Stress, Pathway Profiling
HCS237	Phospho-c-jun (Ser73) Assay Kit	Cellular Stress, Cytotoxicity, Cell-Based Kinase Screening

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Cellular Signaling

Catalogue Number	Application	Description
HCS223	p53 Assay Kit	DNA Damage and Repair
HCS230	Phospho--ERK1/2 (Thr185/Tyr187) Assay Kit	Cell-Based Kinase Screening, GPCR Profiling, Cellular Stress
HCS231	p38 MAPK Assay Kit	Cell-based Kinase Screening, Cell Signaling, Cellular Stress
HCS234	p21 Assay Kit	Cyclin-Dependent Kinase, Inhibition, DNA Damage, Cellular Stress
HCS235	p53 abd p21 Assay Kit	Cell Cycle, DNA Damage, Cellular Stress, Pathway Profiling
HCS237	Phospho-c-jun (Ser73) Assay Kit	Cellular Stress, Cytotoxicity, Cell-Based Kinase Screening
HCS239	α-Catenin Assay Kit	Cell Signaling, Wnt Pathway Screening, Cellular Differentiation

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Staining & Buffer Toolkits

Catalogue Number	Application	Description
HCS214	HCS Toolkit for Mouse Primary Antibodies	Custom Assay Development, Reagent Standardization for Screening
HCS215	HCS Toolkit for Rabbit Primary Antibodies	Custom Assay Development, Reagent Standardization for Screening

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