PhosphataseProfiler
Protein phosphatases are rising in interest as a relevant, druggable target class. As with kinases, inhibitor specificity is a major challenge for discovery scientists. PhosphataseProfiler, Millipore’s phosphatase specificity testing service, offers a robust and convenient way to rapidly generate inhibition profiles for lead phosphatase inhibitors.
Reversible protein phosphorylation is a key component of cellular regulation and homeostasis. The complementary actions of kinases and phosphatases control numerous biological processes including gene transcription, insulin signaling and synaptic transmission.
Now featuring 21 key protein phosphatases for selectivity profiling of novel chemical entities, the growing PhosphataseProfiler panel represents your best choice to aid in the search for phosphatase targeted drugs. PhosphataseProfiler is ideal for profiling lead compounds, chemical arrays, focused libraries and, like KinaseProfiler, has a quick 2-week turnaround time.
Targets Available in PhosphataseProfiler:
| CD45 | PTP MEG1 |
| DUSP22 | PTP MEG2 |
| HePTP | PTPN22 |
| LMPTP-A | RPTPµ |
| LMPTP-B | SHP-1 |
| MKP5 | SHP-2 |
| PP1α | TCPTP |
| PP2A | TMDP |
| PP5 | VHR |
| PTP-1B | YopH |
| PTPβ |  |
DiFMUP Phosphatase Assay Principle
Millipore’s PhosphataseProfiler assay utilizes DiFMUP (Molecular Probes) – 6,8-difluoro-4-methylumbelliferyl phosphate – as a universal substrate. This molecule has low fluorescence intensity in its non-hydrolyzed form. Removal of the phosphate group yields a highly fluorescent product.
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