Discover how your compound is affecting signal transduction pathways
Many important drug target classes either trigger signal transduction (e.g., cell surface receptors like GPCRs and Receptor Tyrosine Kinases) or directly participate in the transduction of the signal (e.g., intracellular kinases like Erk kinase). Signaling pathway analysis enables you to identify how signaling nodes, signal redundancy and feedback loops may affect the desired activity of a compound inhibitor, providing complementary data to target-specific profiling and biochemical assays.
Unique, Biologically Relevant Pathway Analysis
Unlike other pathway analysis services that involve engineered, reporter cell lines or the use of large amounts of sample lysates, Millipore’s SignalProfiler Services provides analysis on a cell or tissue that is appropriate for your research interests, thus bridging a gap between a compound’s in vitro biochemical activity with its more relevant cellular activity.
Benefits of SignalProfiler Services:
- Quantifies changes in phosphorylation of multiple pathway proteins.
- Utilizes Millipore’s MILLIPLEX MAPmates™ immunoassays, which are based on Luminex® xMAP® technology - the most trusted platform for multiplex protein quantification.
- Multiplexed analysis of key pathways including receptor tyrosine kinase (RTK) pathways, MAPK, Akt/mTOR, p38/SAP, and Src kinase.
- Carefully selected panels match your research interests:
- Oncology
- Inflammation
- Metabolic Disease, Diabetes, Obesity
How To Order Services:
- Choose from our two Service Options
- Fill out quote request form (download at the Drug Discovery and Development Portal): We'll send you the quote and guidelines as to how to set up your experiment and handle your cells
- Perform your cell-based assay*: You choose your cells and compounds.
- Prepare your samples: Order a Cell Lysis Kit, prepare lysates and freeze samples.
Cells should be lysed in Millipore’s MILLIPLEX MAP Lysis Buffer (item# 43-040).
- Send us your samples and purchase order
*For SignalProfiler FlexLab Services, we provide cell-based assay service for specified/supplied cell lines and supplied compounds.
