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Applications — Research — Antibody Quantitation

Overview

The Guava RapidQuant Human IgG kit eliminates the most tedious and laborious portions of traditional antibody titer methods, while providing highly accurate, reproducible results in a simple, robust assay. Whether you’re screening for high producing clones or quantifying antibody levels for production, the Guava RapidQuant Human IgG kit is the solution for efficient antibody quantitation: no washing steps, no dilution of typical hybridoma supernatants, no degradation of colorimetric readouts, no need to worry about assaying for each human IgG subtype. All these problems are eliminated in the Guava RapidQuant Human IgG kit without sacrificing accuracy.

		Representative standard curve for the Guava RapidQuant Mouse lgG kit demonstrates its excellent linearity and reproducibility.
		Guava RapidQuant Mouse lgG kit is accurate and precise across a broad concentration range. Various commercial antibodies of subtypes lgG1, lgG2a and lgG2b were diluted to specified concentrations (as determined by A280) and assayed with the RapidQuant Mouse lgG kit. There was excellent correlation between Guava values and the predicted values across the range of assay.
		Guava RapidQuant Mouse lgG kit results are stable long after sample preparation. Plates may be prepared, refrigerated, and read the next day, a convenient feature when the procedure finishes near the end of the day.

	Available reagent kits
		Human IgG Kit		Mouse IgG Kit
	Specificity	IgG1, IgG2, IgG3, IgG4		IgG1, IgG2a, IgG2b, IgG3*
	Dynamic Range	0.5-20 µg/mL		2.5-40 µg/mL
	Kit Size	100 tests		100 tests
	*Detection is lower for IgG3 than for other mouse IgG subtypes

Features and Benefits

• Assay

• Samples

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