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Applications — Manufacturing — Cell Counting & Viability

Overview

Guava ViaCount provides a more realistic assessment of cell viability by measuring a significant number of cells as apoptotic that trypan blue counts as viable, giving engineers an opportunity to to maintain cell viability and productivity by altering cell culture conditions earlier in their process.

Because Guava PCA and associated reagents operate in one-third to one-fifth the time of microscopy and flow cytometry, respectively, the Guava systems provide convenient, accurate, and rapid in-process cell viability measurements in systems under apoptosis-inducing stress.

The Guava ViaCount Assay is fast becoming the new standard for viability and cell counting. In this simple no-wash, mix-and-read assay, you can:

• Accurately obtain absolute total cell counts
• Perform viability assessments
• Determine apoptotic percentages

- all from tiny samples. You’ll enjoy greater accuracy, reproducibility, and speed.

Features and Benefits

• Assay

       •  Simple no-wash, mix-and-read procedure
       •  Counts up to 10 times faster than manual methods
       •  More reproducible than traditional tests

• Samples

       •  Uses small samples in tubes or a 96-well plate
       •  Handles low-density and small-volume cell samples
       •  Works with adherent or suspended cells and mammalian and insect cells
The ViaCount Assay analyzes cells based on differential permeabilities of two DNA-binding dyes. The nuclear dye stains only nucleated cells, while the viability dye brightly stains dying cells. Debris is excluded from results based on negative staining with the nuclear dye.

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The blue population of cells show a significant amount of annexin V staining (bottom plot), indicating that intermediate levels of staining with the viability dye correlates with apoptosis.	Increased reproducibility in ELISPOT assays when using Guava ViaCount assay for cell counting.

Guava ViaCount assay provides accurate cell counts. Comparisons with an impedance counter and manual trypan blue hemocytometer counting indicated greater linearity and accuracy over a rang of cell concentrations. Expected cell counts were determined using an FDA-cleared reference counting bead method.

Additional ViaCount Reagents; For dilute, non-mammalian and clumpy cells

• ViaCount Flex Reagent

       •  Low density cell samples that are too dilute to be counted with standard hemacytometers
       •  Cell lines that stain heterogeneously (facilitates gate settings by providing tighter staining patterns)        » Learn More...

• ViaCount Flex Reagent for Challenging Samples

       •  Non-mammalian cell lines (such as SF9 insect cells) and other cell lines with unusual culture conditions        » Learn More...

• Guava ViaCount Cell Dispersal Reagent

       •  Uses enzymes to gently disaggregate clumped cells in suspension, improving the accuracy and precision of cell counts.        » Learn More...

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