Applications — Research — Cell Counting & Viability
Overview
Cell counting and viability assessments can be used in a variety of applications, such as cytotoxicity studies, PBMC counting, rapid apoptosis assessment, and increasing reproducibility in cell-based assays.The Guava ViaCount Assay is fast becoming the new standard for viability and cell counting. In this simple no-wash, mix-and-read-assay, you can accurately obtain absolute direct total cell counts without the need for reference beads. Additional benefits include viability assessments, and determine apoptotic percentages— all from tiny samples. You’ll enjoy several advantages over traditional methods, including greater accuracy, reproducibility, and speed.
Features and Benefits
- Assay
• Counts up to 10 times faster than manual methods
• More reproducible than traditional tests
- Samples
• Handles low-density and small-volume cell samples
• Works with adherent or suspended cells and mammalian and insect cells
The ViaCount Assay analyzes cells based on differential permeabilities of two DNA-binding dyes. The nuclear dye stains only nucleated cells, while the viability dye brightly stains dying cells. Debris is excluded from results based on negative staining with the nuclear dye.
| The blue population of cells show a significant amount of annexin V staining (bottom plot), indicating that intermediate levels of staining with the viability dye correlates with apoptosis. | Increased reproducibility in ELISPOT assays when using Guava ViaCount assay for cell counting. |
Additional ViaCount Reagents; For dilute, non-mammalian and clumpy cells
- ViaCount Flex Reagent
• Cell lines that stain heterogeneously (facilitates gate settings by providing tighter staining patterns) » Learn More...
- ViaCount Flex Reagent for Challenging Samples
- Guava ViaCount Cell Dispersal Reagent
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