Millipore - Cell Cycle

Applications — Research — Cell Cycle

Overview

Cell cycle regulation is critical to cell survival, as it governs the repair of genetic damage and the prevention of uncontrolled cell division.

The Guava Cell Cycle Assay uses the nuclear DNA stain, propidium iodide (PI) to measure G0/G1, S, and G2/M phase distributions. Resting cells (G0/G1) contain two copies of each chromosome. Cycling cells synthesize chromosomal DNA (S phase), which results in increased fluorescence intensity. When all chromosomal DNA has doubled (G2/M phase), cells fluoresce with twice the intensity of the initial population.

Whether you are exploring the mitotic pathway or evaluating novel compounds for cancer therapies, you will appreciate the ease and convenience of doing cell cycle studies right in your lab.

Features and Benefits

Assay

       • Staining and data acquisition in 1-2 hours
       • All-in-one reagent makes preparation easy
       • Direct, absolute counts and percentages

Samples

• Tube and 96-well plate protocol
• Works with adherent and suspended cells


Guava Cell Cycle displayed using Modfit™, a curve fitting algorithm applied for detailed assessment of the different phases.


All of the necessary functions for visualizing and analyzing data are presented on one screen and include a gating dot plot (A), four-marker histogram (B) and statistical table (C), as well as data analysis functions (D) such as exporting and printing.

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Applications — Research — Cell Cycle

Overview

Features and Benefits

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