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Applications — Research — Chemokine Analysis

Overview

Chemokines play critical roles in a number of cellular processes. They are involved in leukocyte trafficking and activation, mediated by 7-TM chemokine receptors. They are also widely expressed on immune system cells, and can be important indicators of autoimmune disorders and HIV infections. Rapid assessments of chemokine receptor expression are useful for a variety of applications, including infectious disease and inflammation research.

Millipore offers FlowCellect Chemokine Receptor Surface Expression Quantification Kits designed to provide rapid, sensitive assessments in many different cell types, creating a new standard in chemokine receptor quantification. FlowCellect kits use fluorescence detection, coupled with positive and negative control cells, to avoid the hazard and expense of traditional radioligand binding assays.

Control Cells

In addition to optimized antibodies, buffers, and fixatives, FlowCellect Chemokine Receptor Surface Expression Quantification kits include positive and negative control cells to help calculate and confirm your results. These cells are frozen samples of Millipore’s ChemiScreen™ myeloid cell lines; wild-type cells serve as the negative control, while cells from the clonal stable cell line that over-expresses the relevant chemokine receptor are used as the positive control. All cell lines have been fully char­acterized using pharmacology techniques, such as radioligand binding, to give you complete confidence in your data.

Features and Benefits

• Assay

       •  Includes pharmacologically characterized positive and negative control cells
       •  Contains everything you need to run 100 samples
       •  Same accuracy as radioactive assays, without the danger

• Samples

       •  Designed for human cells

The histogram on the far right (blue) represents the positive control cells, the histogram in the middle (green) represents the native cell line (Jurkat T cells), and the histogram on the far left (red) represents the negative control cells.

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