8 Peak Rainbow Beads

Instrument Accuracy

Consistent sample results across an entire plate with sample mixing

• To ensure that cells stay in suspension during sampling, a rotating, variable- speed mixing paddle with auto-cleaning is used between each well so there is no need for manual resuspension after loading the plate.

Evaluation of well-to-well consistency with sample mixing for a 96-well plate.
Sample concentrations were within acceptable range for all samples within the plate.
easyCheck beads were diluted at 1.0x10e6 and placed into all wells of a 96 well plate.
The plate was then spun at 150xg for 5 minutes to mimic settling.
Each well was acquired and concentrations were determined.
Data show the concentrations for each well (solid line) as well as +/- 10% values (dotted lines)

• The mixing feature virtually eliminates carryover between samples and mixes more efficiently than shaking plate holder formats. Data shown below confirms that carryover is minimized with this format

	Plate 1	Plate 2	Plate 3	Plate 4	Plate 5
Carryover	0.15% ±	0.17% ±	0.21% ±	0.23% ±	0.22% ±
Percentage	0.01	0.17	0.08	0.07	0.04

Consistent cell counts for each channel

Scatter resolution for 6-color staining

Immunological Phenotyping-6 Color Assay

HEK-293 cells transfected with GFP (green) or untransfected (grey).

5 decade dynamic range enables simultaneous detection of bright and dim fluorochromes

• High calibration mode enhances the detection of very dim fluorochromes, as it improves the signal to noise ratio, even in the red and far-red (APC) channel.
• Positive and negative populations are detectable in the same scale, even for ultrabright fluorochrome dyes or fluorescent proteins, as shown here.

Software templates for simple acquisition and analysis

• InCyte™ templates are new applications-specific modules with unique features that can be customized to your preferences for analysis. These modules are available for new and existing FlowCellect™ applications.

Pre-calibrated instruments that need no adjustment

• Every guava easyCyte™ instrument manufactured now has optimized calibrated performance to ensure instrument to instrument reproducibility and performance.
• No user adjustments are necessary, as laser and optical filter alignments are fixed with buffers in place to reduce the effects of vibration transference from internal mechanisms or external sources.

Transfer methods and setting between any guava easyCyte, even for systems with different optical layouts

• The ability to transfer instrument settings and methods between instruments allows comparisons of data between instruments and cross-site validation studies.
• Fully-adjustable instrument settings on guava easyCyte™ flow cytometers and GuavaSoft™ software allow the user to optimize, save, and retrieve acquisition settings for SSc and all fluorescent channels (except FSc and NIR2, available late 2012) and display templates.

Over 14 years of experience in Microcapillary Flow Cytometry

• 5,798,222 – Apparatus for monitoring substances in organisms (1998)
• 6,403,378 – Cell Viability assay reagents (2002)
• 6,710,871 and 6,816,257 – Method and apparatus for detecting microparticles in fluid samples (2004)
• 7,320,775 – Exchangeable flow cell assembly with a suspended capillary (2008)
• 7,410,809 – Particle or cell analyzer and method (2008)
• 8,184,271 and 7,847,923 – Differentiation of flow cytometry pulses and applications (2010)
• The InCyte analysis and guava RapidQuant Human and Mouse IgG Assays are the subjects of pending patent applications. (pending as of 2012)

Quality control of daily performance

flx_root