Two-parameter analysis can achieve target-specific detection of phosphorylation, and eliminates false positives in a mixed cell population. In an unstimulated system, only total H2A.X expression is seen (97.22%) in A. Upon treatment with 100 µM Etoposide for 2 hours, simultaneous measurement of both total and phospho H2A.X confirms target specificity (2.09% to 97.15%) B.
Examination of cell signaling pathways and monitoring their activation status have been extremely important for researchers to understand the detailed mechanisms of cellular functions and the cause of various diseases. Many signal transduction pathways have been implicated to lead to multiple outcomes such as apoptosis, cell differentiation, cell growth and cell proliferation, all of which have been extensively studied for the treatment of various cancers and autoimmune diseases.
Features:
Wide choice of kits with an optimized combination of antibodies for common signal transduction pathways which require no compensation.
Simplifies usage of multi-parameter flow cytometry for study of multiple pathways in a mixed cell population.
Eliminates false positives by use of activation status-specific and phospho-specific antibodies.