Analyzed Dual Parameter Data: Dot plots depicting PBMCs activated with 5µg/mL PHA (phytohemagglutinin) for 2 days and stained using FlowCellect™ CD4 T Cell FAS Kit. Dot plots show the percentage of positive cells for CD4 T cells as a percentage of CD3 T cells with and without PHA treatment. The histogram depicts the level of FAS/CD95 expression on CD4 T cells. PHA treatment upregulates the expression of FAS/CD95 on the CD4 T cells. The gating was set up on an unstimulated control sample stained with isotype control and applied to the stimulated samples.
FlowCellect kits are EMD Millipore’s proprietary multiparameter flow cytometry kits for the analysis of cellular events and/or cell phenotypes. Each kit has unique combination of directly conjugated antibodies, and/or fluorescent dyes and protein reporters to monitor changes in protein expression and posttranslational modification. The kits also contain complete buffer sets, and protocols. They are fully optimized for “plug-and-play” cellular analysis on guava and other flow cytometers.
Advantages:
Multiplex detection with high reproducibility
No need for assay development or optimization; includes optimized antibodies and buffers
Enables walk-up use for the performance of complex multiparameter analysis
Advance your flow cytometry analysis with EMD Millipore’s growing selection of directly conjugated primary antibodies. We have expanded our Milli-Mark Conjugated Antibodies portfolio to include the most relevant CD markers used in the immunology field today. The Milli-Mark conjugated antibodies are specifically designed, optimized and validated for flow cytometry applications