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SNAP i.d. Protein Detection System

Protocol Overviews

(1) Blot Holder Assembly

1. Open Blot Holder
2. Web Flow Distributor
3. Place Blotted Membrane

3. Place Spacer
4. Close Blot Holder
4. Turn over Blot Holder


(2) Immunodetection


1. Open Lid
2. Insert Blot Holder
3a. Add Blocking Solution
3b. Turn on Vacuum




4. Turn off Vacuum
5a. Add Primary Antibody
5b. Incubate 10 minutes
5c. Turn on Vaccum
6a. Add wash solution (3x)
6b. Turn off Vacuum
(Repeat Steps 4-6 for 2° Ab



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SNAP i.d.
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