SNAP i.d. Protein Detection System
Protocol Overviews
(1) Blot Holder Assembly
1. Open Blot Holder | 2. Web Flow Distributor | 3. Place Blotted Membrane |
3. Place Spacer | 4. Close Blot Holder | 4. Turn over Blot Holder |
(2) Immunodetection
1. Open Lid | 2. Insert Blot Holder | 3a. Add Blocking Solution 3b. Turn on Vacuum |
4. Turn off Vacuum | 5a. Add Primary Antibody 5b. Incubate 10 minutes 5c. Turn on Vaccum | 6a. Add wash solution (3x) 6b. Turn off Vacuum (Repeat Steps 4-6 for 2° Ab |
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