Immunodetection Techniques
Immunodetection techniques describe any application in which the antibody: antigen interaction is analyzed. Immunodetection depends greatly on the specificity of each antibody for its antigen and the optimization of assay conditions for detection and analysis. We provide you with the complete solution by offering the highest quality of antibodies and reagents along with the most comprehensive guidance on immunodetection techniques in life science research.Snap i.d. Protein Detection System
The SNAP i.d. Protein Detection System revolutionizes the quality of your blots every time — in record time! ...
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Western Blotting
Since its introduction in 1979 (Towbin et al., 1979), protein blotting has become a routine tool in research laboratories...
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Western Blot Reagents
Immobilon Western Substrates are optimized for high sensitivity and low background over a broad dynamic range at a considerably lower cost than other chemiluminescent reagents...
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Western Blotting Protocols
There are two types of protocols for immunodetection: Standard and rapid.
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Immunoprecipitation
It is possible to use antibody-antigen precipitation (immuoprecipitation) to isolate a specific antigen from complex protein mixtures such as cell or tissue lysates.
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Immunopreciptation Kits
Catch and Release® Reversible Immunoprecipiation System (Cat.No 17-500) overcomes many of the limitations associated with conventional IP...
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Immunoprecipitation Protocols
Immunoprecipitation is a widely used method to purify specific proteins from complex samples such as cell lysates or extracts. Step-by-step procedures...
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Immunostaining Techniques
Immunohistochemistry/immunocytochemistry is the demonstration of a tissue or cellular constituent in situ by detecting specific antibody-antigen interactions where the antibody has been tagged with a visible label...
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IHC Select Detection System
Millipore's Premium IHC Select® Detection Systems offer the highest quality staining of formalin-fixed, paraffin-embedded tissues...
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Immunostaining Protocols
Step-by-step procedures...
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Flow Cytometry
Flow Cytometry is a powerful multi-parametric single cell analysis platform with wide applications in basic research and clinical medicine for the diagnosis, isolation and study of specific cell types and disease conditions...
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Flow Cytometry Protocols
Step-by-step procedures...
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ELISA
Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies with the sensitivity of simple enzyme assays, by using antibodies or antigens coupled to an easily assayed enzyme that possesses a high turnover number...
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ELISA Protocols
Step-by-step procedures...
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Elispot
MultiScreenHTS filter plates are available with validated Immobilon®-P (PVDF) membrane for superior results...
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Elispot Protocols
Step-by-step procedures...
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Radioimmunoassay (RIA)
Radioimmunoassay (RIA) was the first of many immunoassay techniques developed to analyze nano molar and pico molar concentrations of the hormones in biological fluids. Berson and Yallow of Albert Einstein School of Medicine initially described the method to determine insulin levels in human plasma in early sixties...
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Multiplex Immunoassay
The rapidly growing knowledge base in drug discovery and protein research has placed increased pressure on researchers to quickly gain a greater understanding of protein-protein interactions, cell signaling pathways, and markers of metabolic processes...
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Assay Development
cUpstate's high quality reagents for assay development support life science research labs globally.
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