• Login
|
• Register
|
• My Account
|
• Shopping Cart
|
• Help

PureProteome™ Nickel Magnetic Beads

Looking for a better way to purify your His-tagged proteins?

Advantages No lysate clearing: Crude cell lysates are processed by magnetic beads. Fast processing time: Entire protocol is completed in less than one hour. High capacity: Bind 5-20mg of protein per mL of settled beads. High affinity: Binding can take place in buffer containing <5mM EDTA. Flexible scale: Varying the bead quantity allows different protein yields. Flexible elution: native or denatured proteins. Automatable: Hands-free screening process.

Click for data sheet

Protocol

(1) Mix washed beads with lysate and incubate for 30 minutes.
(2) Collect beads using Magna GrIP™ rack
(3) Aspirate unbound fraction.
(4) Remove tube from rack. Resuspend beads in 0.5 mL wash buffer and incubate for one minute.
(5) Collect beads using Magna GrIP rack.
(6) Aspirate contaminants (Repeat wash step two more times).
(7) Remove tube from magnetic rack and resuspend beads in 100µL elution buffer. After 2 minute incubation, collect beads using Magna GrIP Rack. Remove purified his-tagged protein. Repeat elution step.

High Purity

Compared with other magnetic purification systems, Proteome Nickel Magnetic Beads offer comparable yield while offering high purities.

Coomassie Blue stained SDS-PAGE gel of purified polyhistidine tagged 24kDa protein from 1mL of E.coli culture using Millipore and competitor magnetic beads. Lane 1: MW standards, lane 2: starting lysate, lanes 3-6: purified protein using competitor beads, lane 7: purified protein using Millipore beads.

High Capacity and High Affinity

Millipore PureProteome Nickel Magnetic beads are manufactured using a patented process which provides magnetic beads with high capacity and high affinity. This permits protein binding to take place even in buffers containing low concentrations of EDTA.

Coomassie Blue stained SDS-PAGE gel of purified polyhistidine tagged 38kDa protein from E.coli lysate in presence of 2mM EDTA using Millipore and competitor magnetic beads. Lane 1: MW standards, lane 2: starting lysate, lanes 3-12: purified protein with (E) and without (C) 2mM EDTA.

Ordering Information

Item	Catalogue Number
PureProteome Nickel Magnetic Beads, 2 x 1mL	LSKMAGH02
PureProteome Nickel Magnetic Beads, 10 mL	LSKMAGH10

Magnetic Bead Platforms		Catalogue Number
Magna ChIP Protein A (buffers, spin filters)	22 assays	17-610
Magna ChIP Protein G (buffers, spin filters)	22 assays	17-611
EZ- Magna ChIP A (complete, includes controls)	22 assays	17-408
EZ-Magna ChIP G (complete, includes controls)	22 assays	17-409
Magna GrIP Rack	8 sample holder	20-400

Got questions ?

We're always ready to help. Visit http://www.millipore.com/support to learn more.
Discover our other tools for sample preparation and immunodetection at our new Learning Center.

// this function fires when the user has clicked on a tab var onTabClicked = function(tabIndex, obj) { window.location.href = getBaseURL() + "#" + tabIndex; AntibodiesNavigatorPanels.setSelectedIndex(tabIndex); } mainSimpleTabs=new SimpleTabs("find_antibodies", 0, onTabClicked); AntibodiesNavigatorPanels.addNavigatorPanel(new NavigatorPanel("primary_antibodies_form", "primary_antibodies_filter", "/pages/microsites/antibodies/primary_antibodies_filter.jsp?navigatorPanelSet=AntibodiesNavigatorPanels", true)); AntibodiesNavigatorPanels.addNavigatorPanel(new NavigatorPanel("secondary_antibodies_form", "secondary_antibodies_filter", "/pages/microsites/antibodies/secondary_antibodies_filter.jsp?navigatorPanelSet=AntibodiesNavigatorPanels")); AntibodiesNavigatorPanels.setSelectedIndex(getTabIndex()); AntibodiesNavigatorPanels.populateFilters(); mainSimpleTabs.setTab(getTabIndex()); if (disableToggle) { $("toggleAdv").style.display = "none"; } AntibodiesNavigatorPanels.getNavigatorPanel(0).setToggleImageId("toggleAdv"); AntibodiesNavigatorPanels.setShowAll(true);

• Primary
• Secondary

Find Primary Antibodies

Enter a keyword and/or search criteria below. Or you may also use selections below to browse specific antibodies for your needs.

Keyword Gene Symbol UniProt Number Entrez Gene Number

Advanced Options

---

Find Secondary Antibodies

Select the host, conjugate, and species, and then press Search to find the secondary antibodies for your research needs.

Product News & Releases

• New Product Guide to Millipore’s Antibodies and Kits - Free reference book for immunoassay and protein research application
• New OxyELISA™ Kit Provides Quantitative Measure of Oxidative Stress - High throughput kit improves specificity and shortens assay time
• PureProteome™ Nickel Magnetic Beads from Millipore
• Millipore Launches SNAP i.d.™ Protein Detection System

Featured Products

• Catch and Release Reversible Immunoprecipitation system
• Protein G Agarose Fast Flow
• Immobilon-FL Transfer Membrane
• Immobilon Chemiluminescent HRP Substrate
• Amicon Ultra
• Ultrafree MC
• Anti-NeuN, Marker for Post-Mitotic Neurons
• Anti-Tyrosine Hydroxylase
• Anti-Actin (Clone C4)
• Anti-Cytokeratin 5, 6, Clone D5/16B4

Pathways