PureProteome™ Nickel Magnetic Beads

Looking for a better way to purify your His-tagged proteins?

Milipore's new PureProteome Nickel Magnetic Beads provide researchers with a powerful bead system to help purify polyhistidine-tagged recombinant proteins faster, easier, and better than every before.

Advantages

No lysate clearing: Crude cell lysates are processed by magnetic beads.
Fast processing time: Entire protocol is completed in less than one hour.
High capacity: Bind 5-20mg of protein per mL of settled beads.
High affinity: Binding can take place in buffer containing <5mM EDTA.
Flexible scale: Varying the bead quantity allows different protein yields.
Flexible elution: native or denatured proteins.
Automatable: Hands-free screening process.

Click for data sheet

Protocol

Just bind, wash, and elute !

1. Mix washed beads with lysate and incubate for 30 minutes.

2. Collect beads using Magna GrIP™ rack

3. Aspirate unbound fraction.

4. Remove tube from rack. Resuspend beads in 0.5 mL wash buffer and incubate for one minute.

5. Collect beads using Magna GrIP rack.

6. Aspirate contaminants (Repeat wash step two more times).

7. Remove tube from magnetic rack and resuspend beads in 100µL elution buffer. After 2 minute incubation, collect beads using Magna GrIP Rack. Remove purified his-tagged protein. Repeat elution step.

High Purity

Compared with other magnetic purification systems, Proteome Nickel Magnetic Beads offer comparable yield while offering high purities.

Coomassie Blue stained SDS-PAGE gel of purified polyhistidine tagged 24kDa protein from 1mL of E.coli culture using Millipore and competitor magnetic beads. Lane 1: MW standards, lane 2: starting lysate, lanes 3-6: purified protein using competitor beads, lane 7: purified protein using Millipore beads.

High Capacity and High Affinity

Millipore PureProteome Nickel Magnetic beads are manufactured using a patented process which provides magnetic beads with high capacity and high affinity. This permits protein binding to take place even in buffers containing low concentrations of EDTA.

Coomassie Blue stained SDS-PAGE gel of purified polyhistidine tagged 38kDa protein from E.coli lysate in presence of 2mM EDTA using Millipore and competitor magnetic beads. Lane 1: MW standards, lane 2: starting lysate, lanes 3-12: purified protein with (E) and without (C) 2mM EDTA.

Ordering Information

Item Catalogue Number

PureProteome Nickel Magnetic Beads C77625

Magnetic Bead Platforms Catalogue Number

Magna ChIP Protein A (buffers, spin filters) 22 assays 17-610

Magna ChIP Protein G (buffers, spin filters) 22 assays 17-611

EZ- Magna ChIP A (complete, includes controls) 22 assays 17-408

EZ-Magna ChIP G (complete, includes controls) 22 assays 17-409

Magna GrIP Rack 8 sample holder 20-400

Item	Catalogue Number
PureProteome Nickel Magnetic Beads	C77625

Magnetic Bead Platforms	Catalogue Number
Magna ChIP Protein A (buffers, spin filters)	22 assays	17-610
Magna ChIP Protein G (buffers, spin filters)	22 assays	17-611
EZ- Magna ChIP A (complete, includes controls)	22 assays	17-408
EZ-Magna ChIP G (complete, includes controls)	22 assays	17-409
Magna GrIP Rack	8 sample holder	20-400

Got questions ?

We're always ready to help. Visit http://www.millipore.com/support to learn more.

Discover our other tools for sample preparation and immunodetection at our new Learning Center.

Rechercher des anticorps primaires

Entrez un mot-clé et/ou un critère de sélection ci-dessous. Vous pouvez également utiliser les menus ci-dessous pour naviguer et trouver les anticorps spécifiques qui répondent à vos besoins.

Rechercher des anticorps secondaires

Sélectionnez l'hôte, le conjugué et les espèces, puis cliquez sur "Recherche" pour trouver les anticorps secondaires qui répondent à vos besoins.

Rechercher des kits et des essais

Entrez un mot-clé et/ou un critère de sélection ci-dessous. Vous pouvez également utiliser les menus ci-dessous pour naviguer et trouver les kits & essais spécifiques qui répondent à vos besoins.

Nouveaux produits et nouvelles publications

» Tout afficher

1. Mix washed beads with lysate and incubate for 30 minutes.
2. Collect beads using Magna GrIP™ rack
3. Aspirate unbound fraction.
4. Remove tube from rack. Resuspend beads in 0.5 mL wash buffer and incubate for one minute.
5. Collect beads using Magna GrIP rack.
6. Aspirate contaminants (Repeat wash step two more times).
7. Remove tube from magnetic rack and resuspend beads in 100µL elution buffer. After 2 minute incubation, collect beads using Magna GrIP Rack. Remove purified his-tagged protein. Repeat elution step.