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Protein Separation and Transfer

Separating and identifying protein molecules has become routine with the use of gel electrophoresis separation and immobilization to membranes (western blotting), especially in the areas of protein characterization and validation of biomarkers. We provide essential components for your gel and blotting analyses. From cell and tissue lysates, to electrophoresis buffers and blotting membranes, to the reagents used to visualize your results, we can help you identify and characterize protein molecules important to your research.

Electrophoresis

Immunoblotting procedures combine the resolution of gel electrophoresis with the specificity of antibody detection...
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Membrane Selection

Polyvinylidene fluoride (PVDF) and nitrocellulose are the two membrane types most commonly used in Western blotting applications..
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Protein Binding

PVDF is an inherently hydrophobic polymer and will not wet-out in aqueous solutions. ..
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Protein Transfer

The process of transferring proteins from a gel to a membrane while maintaining their relative position and resolution is known as blotting...
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Prepare Membrane

PVDF membrane should be washed with deionized water to remove any gel debris. The blot can then be incubated with the blocking solution..
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Transfer and Visualization Protocols

Immobilon Transfer Membranes are robust, versatile PVDF substrates for a variety of applications, including westerns, dot/slot blots, protein sequencing,and MS analysis...
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Western Blotting

Since its introduction in 1979 (Towbin et al., 1979), protein blotting has become a routine tool in research laboratories.
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• Primary
• Secondary

Find Primary Antibodies

Enter a keyword and/or search criteria below. Or you may also use selections below to browse specific antibodies for your needs.

Keyword Gene Symbol UniProt Number Entrez Gene Number

Advanced Options

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Find Secondary Antibodies

Select the host, conjugate, and species, and then press Search to find the secondary antibodies for your research needs.

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