Protein Separation and Transfer
Separating and identifying protein molecules has become routine with the use of gel electrophoresis separation and immobilization to membranes (western blotting), especially in the areas of protein characterization and validation of biomarkers. We provide essential components for your gel and blotting analyses. From cell and tissue lysates, to electrophoresis buffers and blotting membranes, to the reagents used to visualize your results, we can help you identify and characterize protein molecules important to your research.
Electrophoresis
Immunoblotting procedures combine the resolution of gel electrophoresis with the specificity of antibody detection...
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Membrane Selection
Polyvinylidene fluoride (PVDF) and nitrocellulose are the two membrane types most commonly used in Western blotting applications..
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Protein Binding
PVDF is an inherently hydrophobic polymer and will not wet-out in aqueous solutions. ..
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Protein Transfer
The process of transferring proteins from a gel to a membrane while maintaining their relative position and resolution is known as blotting...
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Prepare Membrane
PVDF membrane should be washed with deionized water to remove any gel debris. The blot can then be incubated with the blocking solution..
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Transfer and Visualization Protocols
Immobilon Transfer Membranes are robust, versatile PVDF substrates for a variety of applications, including westerns, dot/slot blots, protein sequencing,and MS analysis...
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Western Blotting
Since its introduction in 1979 (Towbin et al., 1979), protein blotting has become a routine tool in research laboratories.
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Product News & Releases
- New Product Guide to Millipore’s Antibodies and Kits - Free reference book for immunoassay and protein research application
- New OxyELISA™ Kit Provides Quantitative Measure of Oxidative Stress - High throughput kit improves specificity and shortens assay time
- PureProteome™ Nickel Magnetic Beads from Millipore
- Millipore Launches SNAP i.d.™ Protein Detection System
Featured Products
- Catch and Release Reversible Immunoprecipitation system
- Protein G Agarose Fast Flow
- Immobilon-FL Transfer Membrane
- Immobilon Chemiluminescent HRP Substrate
- Amicon Ultra
- Ultrafree MC
- Anti-NeuN, Marker for Post-Mitotic Neurons
- Anti-Tyrosine Hydroxylase
- Anti-Actin (Clone C4)
- Anti-Cytokeratin 5, 6, Clone D5/16B4
