Desalt in a single spin

Buffer exchange using dialysis or diafiltration is often required to make a protein sample compatible with specific downstream analyses. But dialysis or multi-step diafiltration is time-consuming, risks sample loss and can require subsequent concentration. The Amicon® Pro device offers ground-breaking, single-spin diafiltration for simultaneous buffer exchange with concentration. Whether you’re performing this step simultaneously with elution from resin (see Purification section) or whether you’re performing diafiltration only, you’ll experience the same high diafiltration efficiency designed into the device.

Advantages of reducing number of spins for buffer exchange:
  • Sample is not repeatedly diluted and re-concentrated
  • Less chance of protein aggregation
  • Decreased chance of protein precipitation
  • Maintains high protein activity
  • Saves time
Buffer exchange

Diafiltration (Buffer Exchange)

Compare the Amicon® Pro workflow with traditional methods:

Diafiltration
dialysis (cassettes, tubing) + concentration device
0.5 mL centrifugal diafiltration devices
Amicon® Pro Purification System
Process time
Steps required
Sample transfers		17 hours
6
2		45 minutes
4
0		15 minutes
2
0


The gentleness of dialysis with the efficiency of diafiltration

Gentler buffer exchange = greater activity.

Dialysis cassette + concentrator
0.5 mL diafiltration device (3 spin)
Amicon® Pro Purification System
Process time
Recovery
Specific activity
(signal/mg GST-LLP)		16 hours
51%
0.195		50 min.
>95%
0.17		20 min.
>95%
0.199