Protein Sample Preparation: Concentration - Amicon Ultra - Overview

Amicon® Ultra Centrifugal Filters - Overview

Fast and easy protein concentration.

Amicon® Ultra Centrifugal filters provide fast sample processing and promote high sample recoveries, even in dilute samples, through ultrafiltration. The unique features of the Amicon® Ultra centrifugal filters give you the fastest, most efficient concentration for sensitive downstream applications.

Select an Amicon® Ultra Centrifugal Filter by identifying your starting volume:

Concentration and percent recovery using Amicon® Ultra Filters: 4 different devices (Amicon® Ultra-0.5 mL, Amicon® Ultra-2 mL, Amicon® Ultra-4 mL, Amicon® Ultra-15 mL), were tested with four different proteins (3 kDa Cytochrome C, 10 kDa Cytochrome C, 30 kDa BSA and 100 kDa IgG) to determine percent recovery and concentration factor.

Advantages:

True Dead Stop
- Avoids spinning to dryness
- Provides a predictable concentration factor
- No need to calibrate for several samples to run in parallel
Reverse Spin Recovery
- Reverse spin devices enable you to maximize protein recovery without introducing pipetting errors
- Low binding membrane and polypropylene housing for > 90% sample recovery
Vertical membranes
- Aligned with filtrate rather than perpendicular for less clogging, less waste and faster filtration
- Ultra-fast sample processing achieving concentration in as little as 10 minutes
- 25- to 80-fold concentration in a single step
Broad Chemical Compatibility
- Heat-sealed membrane eliminates adhesives and downstream extractables
- Large spectrum of compatibility
- Compatible with pH 1 to 9
Reliable Samples
- Spin precious samples with confidence in one robust, sleek unit that prevents leakage