Protein Sample Preparation: Concentration - General - Microcon

Microcon® Centrifugal Filters

Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel® membrane, Microcon® filters offer:

Typical recoveries of >95%, even for dilute solutions
Reverse spin to maximize recovery, even in the smallest samples
Convenient storage of filtrate or concentrated sample in standard microfuge tube
Concentration factors up to 100X

Microcon® DNA Fast Flow Filter

Optimized for the concentration and recovery of genomic DNA with SDS buffer. The low nonspecific binding characteristics of the membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.

Features:

High recovery for small volumes with reverse spin (concentration factor <20X)
Low-binding Ultracel® membrane
Fast processing

Sample applications:

Recovery of genomic DNA for forensic applications
Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
Removal of primers from amplified DNA
Easy filtrate recovery, exploiting the flat membrane design

Click for Applications Guidelines

	Microcon^® Device
Application	10K	30K	DNA Fast Flow
Peptide and growth factor concentration	•
Protein concentration and desalting of columns eluates	•	•
Protein concentration before electrophoresis or other assays	•	•
Protein removal prior to HPLC	•	•
Purification of macromolecular components found in tissue culture extracts and cell lysates	•	•
Concentration of biological samples (antigens, antibodies, enzymes)		•
Concentration of gDNA with or without SDS buffer		•	•
Concentration and desalting of nucleic acids (single-or double-stranded)	•	•	•
Removal of labeled nucleotides	•	•	•
Removal of labeled amino acids	•	•	•
Removal of primers from amplified DNA		•	•
Removal of linkers prior to cloning		•	•

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Click for Specifications

Maximum initial sample volume	0.5 mL (500 μL)
Typical final concentrate volume	5–50 μL
Maximum relative centrifugal force
Microcon® 10K devices	14,000 × g
Microcon® 30K devices	14,000 × g
Microcon® DNA Fast Flow devices	500 × g
Recovery spin	1,000 × g
Active membrane area	0.32 cm²
Hold-up volume	≤ 10 μL
Dimensions
Diameter	12.3 mm (0.5 in.)
Length (filter device and tube in concentration mode)	45.0 mm (1.8 in.)
Length (filter device and tube in recovery mode)	48.2 mm (1.9 in.)
Materials of Construction
Membrane	Ultracel® low binding regenerated cellulose
Device top	Polycarbonate
Membrane support base	Acetal
Filtrate/concentrate tube	Polypropylene
O-ring	Medical-grade silicone rubber