Water for Endocrine Disrupter Analysis

All the test methods designed to find out if a molecule is an endocrine disrupter and to describe or quantify its effects, require ultrapure water free of endocrine disrupters. Water is used, according to the technique employed, for making buffers, standards for the production of the cell culture media and so on. We will focus, here, on GC and LC/MS applications.

Water quality parameters

The high purity water selected should comply with the LC/MS/MS or GC/MS water quality requirements (See HPLC, UPLC, GC and ICP-MS).

However, besides the well known HPLC and GC contaminants, (TOC, ions, particles, bacteria…), EDC themselves have to be removed from water used for sample, blank, buffers… preparation. If not removed, there is a risk of false positive answers (The suspected molecule found can be due to the molecule tested or to endocrine disrupters that present in the water used for the experimentation).

A final endocrine disrupter polisher (EDS-Pak) has been then specifically designed for Endocrine Disrupter removal.

This Point-of-Use purifier permits a significant removal of EDC, when connected to an ultrapure water system .

Data

In Table 1, results of challenge tests performed on EDS-Pak are presented. It appears that EDS-Pak removes efficiently (from ppb to ppt level), at least 4 of the most famous Endocrine Disruptors compounds. Those experiments have been done by passing 300L of feed water through the EDS-Pak POU.

EDS tested	Feed (ppb)	Product (ppt)	Volume (l)	Flow (l/min)
Bisphenol A	Up to 4	< DL (5 ppt)	300	0.5 — 2.0
Diethyl phtalate	Up to 1.5	< DL (200 ppt)	300	0.5 — 2.0
Di-n-butyl phtalate	Up to 1.5	< DL (200 ppt)	300	0.5 — 2.0
Nonylphenol	Up to 3.3	< DL (100 ppt)	300	0.5 — 2.0

Table 1: Results of challenge tests

	More information Millipore Resources References Generating high-purity water for endocrine disrupter analysis Waterline Club Poster: Challenging organic contaminants in high purity water: persistent organic pollutants and endocrine disrupters Other Resources http://www.epa.gov/endo/pubs/edspoverview/whatare.htm http://openlearn.open.ac.uk/mod/resource/view.php?id=293822 http://www.niehs.nih.gov/health/docs/endocrine-disruptors.pdf http://www3.appliedbiosystems.com/cms/groups/psm_marketing/documents/generaldocuments/cms_039263.pdf http://ec.europa.eu/research/endocrine/projects_ongoing_en.html http://www.epa.gov/scipoly/oscpendo/ Feel free to express your comments Your opinion is important to us. Let us know if this information was useful, or if there was something missing that you'd like to see. Contact us.

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