Pyrogen-free water

Preparing pyrogen-free water using ultrafiltration

Ultrafiltration is a membrane-based technology that removes endotoxins by a filtration process. Ultrafilters are depth filters with nominal weight cut-off ranging from 5 000 Da to 100 000 Da. To remove endotoxins, filters with a molecular weight from 5 KDa to 13 KDa are selected.

Ultrafilters are positioned at the point-of-use, at the outlet of the water purification process. The water delivered is pyrogen-free and does not require autoclaving.

Data shown on Figure 2 demonstrate the efficiency of endotoxin removal by a 13 KDa ultrafilter (BioPak®). This ultrafiltration device is fully efficient over a four month period and large quantities of pyrogens.



Demonstration of endotoxin removal by a 13 KDa ultrafilter (BioPak)

Conclusions

Ultrafiltration is an efficient and simple way to obtain pyrogen-free water on-demand. It avoids autoclaving procedures on water. Pyrogen-water purified using ultrafiltration can be utilized safely for cell culture, including stem cell culture.

The BioPak ultrafilter comes with a certificate guaranteeing endotoxin-free water for four months when a BioPak point-of-use filter is connected to a high purity water purification system, and utilized in normal laboratory conditions.

	More information Literature For further reading, please check out the following literature in the Waterline club. R&D Notebook RD006 – Qualification study of a point-of-use ultrafiltration device for the production of pyrogen-free ultrapure water. S Mabic, D Darbouret, and I Kano.Millipore literature RD006EN00 Application Note - Endotoxin-Free Water for Tissue Culture. E Riché, J Bôle and S Mabic.Millipore literature AB1675EN00 Feel free to express your comments Your opinion is important to us. Let us know if this information was useful, or if there was something missing that you'd like to see. Contact us.

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