Direct Detect® FTIR protein quantitation matches results of amino acid analysis

Amino acid analysis using FTIR protein quantitation
Click to enlarge.
Identical concentration measurements for Lysozyme, Protein A and protein mixtures using Direct Detect® spectrometer and amino acid analysis. Error bars = standard deviations of triplicate sample spots (Direct Detect) or readings (AAA reference method)

Amino acid analysis delivers possibly the most accurate protein quantitation; however, it is expensive and slow if samples are sent to a third party for analysis. If amino acid analysis is performed in-house, it requires time-con¬suming sample manipulation and specialized equipment. The Direct Detect spectrometer provides protein quantitation in agreement with amino acid analysis (AAA), as shown*.

The Direct Detect spectrometer provides universally applicable, less limited and faster protein quantitation that requires minimal amounts of often precious samples. Thanks to its new membrane technology for sample handling and presentation, the Direct Detect™ method is convenient, requires minimal sample preparation and can be used under diverse sample conditions.

* For complete data and experimental details, download application note