FTIR Spectrometer Technology for Protein Quantitation

Direct Detect FTIR Spectrometer protein analysis results
Direct Detect spectrophotometer uses Amide I signal to quantify proteins and peptides. Click image to enlarge.
By measuring amide bonds in protein chains, the system accurately determines an intrinsic component of every protein without relying on amino acid composition, dye binding or redox potential. Accurately analyze components of complex mixtures: proteins, lipids, carbohydrates, and nucleic acids have separable IR spectra.

Advantages of FTIR Quantitation:


How it Works:

Simple to use, even for complex samples.

1. Spots Sample

FTIR Spectrometer protocol - Spot samples on card
  • Apply 2 µL of sample solution (position 2-4) and sample buffer blank (position 1) on the card.

2. Measure (2 min / card)

FTIR Spectrometer measurement protocol
  • Insert the card vertically into the slot in the top of the sampling accessory.
  • The card notch should face towards the center of the instrument (align arrows on card and instrument).
  • Instrument dries samples, then measures each spot.
  • Acquire spectra.

3. FTIR Analysis (quantify protein samples)

  • Statistical data displayed for all 3 samples.
  • User can select which data points to exclude, if any.
  • User has option to print a report of current data, or export as a .CSV file.
  • Compare results to internal Amino Acid Analysis verified BSA standard or create your own standard curve.
  • Assay-free analysis means you can save your standard curves.
Direct Detect Fourier transforms FTIR Spectrometer results

System Components

  • Direct Detect FTIR Spectrometer
  • Assay-Free Card
  • Software