What are customers saying about the Millicell HY cell culture flask?

     
 
Les Henderson, Research Technologist:
"We are using a mouse model to compare cell lines that express an oncogene of interest to a cell line in which the gene of interest has been knocked down. In order to have the statistical power needed for these studies, we use multiple injection sites for which we must grow hundreds of millions of cells. In the past we used standard tissue culture flasks to accomplish this task, which was very time intensive, used a large amount of incubator space, and required much time to harvest and prepare the cells for injection.

Recently, we decided to try Millipore’s HY T-1000 flasks, which reduced the time spent growing cells more than 5-fold. The incubator space requirement to grow these cells decreased from multiple incubators to half an incubator, which was far more manageable with our limited lab space. Additionally, harvesting the cells for injection now took a fraction of the time compared to the standard tissue culture flasks.

With the new flasks, our experiments are proceeding far better than ever before with fewer tumors growing in the knock-down cell lines. Furthermore, after injection, the oncogene expression cell line has a higher take-rate and is growing more aggressively than our prior studies. We believe being able to more quickly harvest our cells, prepare them and inject them into our mice allows for healthier cells and has contributed significantly to our results.” 		 
     
     
 
Customer #2 describes one colleague’s success story:
"If everything goes perfectly with her T75 flask culture, it takes 8 flasks of cells to give the same yield as one 3-layer Millicell HY flask. BUT, according to Denise, the T75 flasks give inconsistent yield, so she actually uses 9 flasks to ensure she gets the same yield as from one 3-layer Millicell HY flask. She says that the Millicell HY flask gives her consistent growth, and that with her cell line and the Millicell HY flask, she can count on 24 million cells from every flask.” 		 
     
     
 
Customer #3 cites top reasons for using Millicell HY cell culture flasks:
“The top reasons why I like using these flasks:
  • Space saver in incubator for when we are growing up cells for a screen.
  • Able to screen more plates per day; easier to manage 15 flasks at a time versus 50!
  • Yield and growth rate comparable to single layer flask.
  • Increases our throughput dramatically."