Millipore's Stable ChemiScreen Ca2+ -Optimized GPCR Cell Lines Provide a Standard Assay Setup and Common Readout for All Receptors
ChemiScreen Ca2+-Optimized GPCR Stable Cell Lines
To meet the demands of high throughput screening and selectivity profiling, our ChemiScreen G Protein Coupled Receptor (GPCR) cell lines are a unique, robust, uniform platform for cell-based functional assays. Endogenous expression of promiscuous G proteins in ChemiScreen cells funnel GPCR signaling to a common calcium readout, regardless of G protein coupling status.
Advantages of ChemiScreen GPCR cell lines:
High functional expression of receptors at the cell surface
Stable cell lines for increased reliability and reproducibility
Single and simple functional readout
Signaling to Ca2+, regardless of G protein coupling status
Identical assay conditions and data analysis for straightforward comparative studies
ChemiBrite Stable Parental and GPCR Cell Lines
Engineered to flash with brilliance upon GPCR activation, our new ChemiBrite cell lines were developed to provide the greatest readout flexibility, including luminescent and fluorescent calcium flux as well as cAMP. Available as either “tool box” reagents (parental line) to develop your own GPCR cell lines or as fully tested cell lines stably overexpressing a GPCR.
Advantages of ChemiBrite Luminescent Assay:
High assay signal
No assay interference with autofluorescent compounds
Non-toxic substrate enables greater flexibility in scheduling assays
