FlexLab Services
Our FlexLab provides you with experience, expertise and the flexibility you require to expand your screening and profiling capacity where you need it. We offer custom cell line development for GPCRs, and our custom assay development team can design unique assays or extend our current services to fit individual needs. Email drugdiscovery@millipore.com to take advantage of FlexLab Services today.
GPCR FlexLab Service can perform a spectrum of cell based functional assays to look at G protein-dependent signaling, including phosphatidylinositol (PI) hydrolysis and cAMP assays. Left panel: Determining the agonist activity of carbachol at M1 receptors expressed in Chem-1 cells by PI hydrolysis. Middle and Right Panels: cAMP is a common readout to determine functional activities of molecules at Gs and Gi/o coupled receptors.
Cell line development
- Cloning and mutagenesis
- Stable lines tailored for user-defined assays
- Ready-to-Assay cells: cryopreserved cells for immediate assays without laborious tissue culture
FlexLab GPCR Services
Custom cell and assay development or bulk manufacturing
- Cell line development
- Fit-for-purpose stable or bulk transiently transfected cells
- Radioligand binding – when you need to quantitate a compound’s affinity
- Saturation (Kd & Bmax) and Competition (Ki determination) assays
- Functional and Inverse Agonist Assays
- GTPγS – well suited for further investigation of Gi/o-coupled receptors
- PI hydrolysis – for analysis of Gq/11-coupled receptors
- cAMP – well suited for examining Gs and Gi/o-coupled receptors
- Secondary assays – for confirmation of hits in downstream or whole cell response assays
- Pathway analysis (e.g., Erk and Creb phosphylation) – common signaling event downstream of GPCRs
- Bulk manufacturing – for high volume use of existing Millipore GPCR products or your reagents developed in-house.
- Membrane preparations
- Frozen, Ready-to-Assay cells
Get Started
- Log in to our Drug Discovery & Development Service Portal
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