IC50Profiler
Our IC50Profiler service enables you to follow up hits identified in a standard KinaseProfiler study by determining an IC50 value for your test compound against the kinase of interest. Our IC50 curves consist of 9 compound concentrations plus blanks, for a total of 20 wells. Compound dilutions are in a half-log series, with the top concentration being selected from the following scale: 100 μM, 30 μM, 10 μM, 3 μM, 1 μM, 0.3 μM, 0.1 μM etc. Your report will include a graphical representation of the data and the estimated IC50 value.
The developmental anti-inflammatory compound, BIRB0796, displays potency at both its primary target, intracellular target, Sapk2a/p38รก (left panel) and the off-target transmembrane tyrosine receptor kinase, Tie2/TEK tyrosine kinase (right panel).IC50Profiler Highlights:
- Select from 300 protein and lipid kinases
- Single point inhibition data or 10-point IC50 curves
- Choice of 10 μM or KM ATP (Custom ATP concentration also available)
- Gold Standard radiometric protein kinase assays
- HTRF Lipid PI3 Kinase assays
- Flexibility to include non-standard solvents or preincubations
- Standard turnaround: 2 weeks or less
Ordering and Service Details