Representative results from PhosphataseProfiler screening of six compounds against a panel of phosphatases shows targeted inhibition of PP1a, PP1b, and PP2A.


PhosphataseProfiler Service

Protein phosphatases are rising in interest as a relevant, druggable target class. As with kinases, inhibitor specificity is a major challenge for discovery scientists. PhosphataseProfiler, Millipore’s phosphatase specificity testing service, offers a robust and convenient way to rapidly generate inhibition profiles for phosphatase inhibitors.

Reversible protein phosphorylation is a key component of cellular regulation and homeostasis, controlling numerous biological processes including gene transcription, insulin signaling and synaptic transmission.
PhosphataseProfiler Highlights:
Targets Available in PhosphataseProfiler
CD45 PTP MEG1
DUSP22PTP MEG2
HePTPPTPN22
LMPTP-ARPTPµ
LMPTP-BSHP-1
MKP5SHP-2
PP1αTCPTP
PP2ATMDP
PP5VHR
PTP-1BYopH
PTPβ
DiFMUP Phosphatase Assay Principle
Millipore’s PhosphataseProfiler assay utilizes DiFMUP (Molecular Probes) – 6,8-difluoro-4-methylumbelliferyl phosphate – as a universal substrate. This molecule has low fluorescence intensity in its non-hydrolyzed form. Removal of the phosphate group yields a highly fluorescent product.
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