Plasmid Purification

Small-scale plasmid purifications (“minipreps”) are required for identifying bacterial clones that contain engineered constructs with the correct insert, correct sequence, and optimal growth properties. High quality miniprep DNA can also be used directly for high throughput sequencing, library construction, RNAi, or other cell-based screens. With over 50 years of experience in membrane filtration, EMD Millipore offers filtration-based plasmid purification tools to increase miniprep throughput without compromising DNA quality, enabling both faster decisions and reproducible data.

Montage Plasmid Miniprep96 Kit
The Montage Plasmid Miniprep96 Kit is a automation-compatible, easy-to-use kit for high throughput, high-purity plasmid or bacterial artificial chromosome (BAC) minipreps. Using a patented separation technology, the Montage Kit follows a simple protocol that eliminates lengthy bind/elute methods and centrifugation steps to yield clean and reproducible DNA in 50% less time than traditional methods. Following lysis, only three short filtration steps are required to prepare 96 DNA samples from each plate



Perform high throughput minipreps using the Montage Plasmid MiniprepHTS Kits:
  1. Following bacterial lysis, transfer lysates from deep well culture block into clearing plate. Clear lysates into Plasmid filter plate using vacuum.
  2. Move Plasmid plate to top of manifold and apply vacuum for 5 to 7 minutes to concentrate sample. Plasmid DNA is retained on the membrane surface while contaminants are filtered to waste. Add wash solution and filter for 3 to 5 minutes.
  3. Add resuspension buffer and collect purified sample by aspiration.
  4. Transfer samples to V-bottom plate for use or storage.
High Yields From Montage Plasmid Miniprep96 Kits*
Host strainPlasmidYield (µg) per well
XL 1-bluepUC192.1 – 6.1
pLH2**3.5 – 9.2
JM109pUC192.4 – 6.4
pLH2**3.5 – 9.1
*Results will vary depending on host strain, plasmid, and protocol conditions.
**pLH2 was derived from pUC19. The 2.0 Kb fragment of Hind III digested I phage was cloned into the Hind III site of pUC19, resulting in a 4.7 Kb plasmid.