Complete Molecular Biology Toolkit for Bacterial Cell Transformation

Transformation (Bacterial)

For successful isolation and propagation of engineered DNA vectors, transformation into competent bacterial cells is a key step. The right strain of competent cells can make the difference between cloning frustration and progressing to the next step. EMD Millipore offers a range of strains and formats to suit every cloning project. Since reliability is critical, we verify the phenotype and purity of each strain and guarantee its transformation efficiency. With more than two decades of experience producing competent cells for protein and molecular biology research, we offer robust performance you can count on.

NovaBlue™ Competent Cells

For routine initial cloning, time-tested NovaBlue Competent Cells are ideal. NovaBlue is a K-12 derivative that offers high transformation efficiency, facilitates plasmid stability, and allows blue/white screening with appropriate plasmids.

Concerned about phage resistance? NovaBlue T1^R is resistant to T1 and T5 phage. And when selecting for pEXPR constructs using Gateway^® Nova pDEST™ vectors or when cloning into pETcoco™ vectors, NovaF- is the right tool for the job.

NovaBlue is a K-12 derivative that offers guaranteed transformation efficiency of >1.5 x 10⁸ cfu/µg, facilitates plasmid stability, and allows blue/white screening with appropriate plasmids.

NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation. Cells are provided in 50 µL volumes to eliminate the need to aliquot, freeze/thaw, or waste partially used vials. This saves time, money, and ensures reliable cell performance.

NovaBlue Competent Cells Transformation Efficiency* Reaction Size Application

GigaSingles™ > 1.0 x 10⁹ cfu/µg 50 µL High-efficiency cloning

Singles > 1.5 x 10⁸ cfu/µg 50 µL Routine cloning

Veggie™ Singles > 1.5 x 10⁸ cfu/µg 50 µL Applications requiring nonanimal-derived materials

Routine cloning

HT96™ > 1.0 x 10⁸ cfu/µg 96 x 20 µL High-throughput cloning

Standard > 1.5 x 10⁸ cfu/µg 50 µL Routine cloning

*Measured as cfu/µg test plasmid

NovaBlue Genotype:
endA1 hsdR17(r_K12-m_K12+) supE44 thi-1 recA1 gyrA96
relA1 lac F’[proA+B+ lacl^qZΔM15::Tn10] (Tet^R)

NovaBlue Competent Cells	Transformation Efficiency*	Reaction Size	Application
GigaSingles™	> 1.0 x 10⁹ cfu/µg	50 µL	High-efficiency cloning
Singles	> 1.5 x 10⁸ cfu/µg	50 µL	Routine cloning
Veggie™ Singles	> 1.5 x 10⁸ cfu/µg	50 µL	Applications requiring nonanimal-derived materials Routine cloning
HT96™	> 1.0 x 10⁸ cfu/µg	96 x 20 µL	High-throughput cloning
Standard	> 1.5 x 10⁸ cfu/µg	50 µL	Routine cloning
Measured as cfu/µg test plasmid NovaBlue Genotype:* endA1 hsdR17(r_K12-m_K12+) supE44 thi-1 recA1 gyrA96 relA1 lac F’[proA+B+ lacl^qZΔM15::Tn10] (Tet^R)

NovaBlue Singles Competent Cells (Catalogue No. 70181)
HT96 NovaBlue Competent Cells (Catalogue No. 71011)
NovaBlue T1^R Singles Competent Cells (Catalogue No. 71318)

Veggie NovaBlue Singles Competent Cells

For cloning applications in which absence of animal-derived components is essential, use Veggie NovaBlue Singles Competent Cells. Like all NovaBlue competent cells, Veggie NovaBlue is a K-12 strain featuring high transformation efficiency, blue/white screening capability (with appropriate plasmids), and mutations in recA and endA, which result in excellent yields of high-quality plasmid DNA. Veggie NovaBlue Singles Competent Cells are supplied with an animal-free prepared SOC medium.

Veggie NovaBlue Singles Competent Cells (Catalogue No. 71251)