Expansion of Cell Cultures for Protein Expression

Growing large numbers of mammalian or insect cells for protein expression is often challenging because of limited incubator space, risk of contamination, extended bench time required for passaging cells, poor yields from culture flasks, and high cost of media and reagents.

EMD Millipore’s Millicell® HY Multilayer Culture Flasks address these challenges. Together with our Calbiochem® cell-culture tested antibiotics, they make selecting and expanding transfected cell cultures easy and reproducible.

Millicell HY (High Yield) Cell Culture Flasks
Depend on Millicell HY cell culture flasks for uniform, consistent cell growth, which enables the amazingly high cell yields provided by these unique flasks. When choosing a multilayer cell culture flask to save space, time and – most importantly – grow healthy cells, trust in EMD Millipore to deliver a flask that outperforms the rest.


Millicell HY Culture Flask Feature:How It Helps You:
Consistent, quality growth environment across all layersBetter cell proliferation and yields than other multilayer flasks
Unique, ergonomic designEasy cell passaging and recovery
No leaking or spillover between layersReduced risk of contamination
Multilayer formatSaves you:
  • Incubator space
  • Time spent passaging cells
  • Cost of reagents

Flask% of Theoretical yield*Flasks required**Volume of medium requiredThe Millicell Benefit
Millicell 3 Layer - 600 cm297%1150 mL
  • Less Media
  • Fewer Flasks
  • Save Incubator Space
  • Time Savings
Brand N T75 - 75 cm2100%8169 mL
Brand N 3 Layer - 500 cm279%1.5189 mL
Millicell 5 Layer - 1000 cm290.5%n/a300 mL
  • Less Media
  • Healthier Cells
  • Cost Savings
Brand C 10 Layer - 1720 cm255%n/a560 mL
* Per unit surface area
** For same cell yield as Millicell HY flask


Antibiotics and Selection Agents
To select cells that have been successfully transfected with DNA carrying an antibiotic resistance marker, choose from our wide variety of high quality Calbiochem cell-culture tested antibiotics. Cited in thousands of peer-reviewed journals, Calbiochem antibiotics are manufactured in a controlled environment and perform with exceptional consistency to ensure the best results, lot after lot. Examples of Calbiochem antibiotics frequently cited in publications:

AntibioticCatalogue Number# of Citations from 2000-2009
G-418 Sulfate, cell culture tested3458106200
Bafilomycin A1, Streptomyces griseus196000460
Hygromycin B, Streptomyces sp., cell culture tested4000522550