Genomic DNA Extraction:
PureGenome™ DNA Extraction Kits for liquid, whole tissues and tissue sections
Generate pure genomic DNA, ready for shearing and library prep. Sixteen micrograms of genomic DNA was purified from 25 mg of mouse heart tissue using the PureGenome™ Tissue DNA Extraction kit. Lane 1: 0.1-12 kbp DNA ladder; lane 2: mouse heart genomic DNA. A260/A280 ratio was 1.8. B. DNA shown in (A) was used to generate an Illumina sequence-compatible NGS library; DNA was first sheared using a Covaris® S220 Ultrasonicator to an average size of 200 bp (red trace). Two microgram of the sheared DNA was used to generate the NGS library using the PureGenome™ NGS Library Preparation Reagents (blue trace). Click image to enlarge.

Click image to enlarge.
Choose the PureGenome™ Tissue DNA Extraction Kit for rapid isolation of total DNA from a variety of solid tissues or the PureGenome™ On-Spot Tissue DNA Kit for extracting DNA from targeted tissue sections (includes formalin fixed paraffin-embedded (FFPE) tissues). Furthermore, you can use these kits to process samples of virtually any biological liquids, such as whole blood, plasma, serum or cells from culture.
High quality DNA isolation from FFPE sample
PureGenome™ On-Spot Tissue DNA Kit was used to isolate total DNA from FFPE tissue sections (normal human liver). An area of ~ 100 mm2 was selected on two human liver FFPE tissue slides. Quantitative PCR was used to detect either a mitochondrial (ND6; purple) or a nuclear-encoded gene (BECN1; blue) from the DNA isolated using the PureGenome™ On-Spot Tissue DNA Kit. Input DNA was 2, 0.2 and 0.02 ng (curves labeled 1, 2 and 3, respectively).