Featured Application Note
Cell Counting and Viability: Precise and Accurate Counts and Viability Measurements Across Multiple Cell Lines Using the Muse™ Cell Count & Viability Assay
In this application note, we show that the Muse
™ Cell Count & Viability Assay provides superior performance to conventional viability and count measurement by Trypan blue exclusion.
The assessment of cell concentration in combination with viability is an important step in the characterization of cell health. Cell viability information can be used for monitoring proliferation rates, optimizing growth conditions and normalizing cell data for further studies and assessing the impacts of cytotoxic compounds. Current methods rely on multiple, sometimes complex instrument platforms to provide these answers, reducing flexibility, limiting the ability to simply obtain comprehensive cell health information and adding increased costs to researchers. Other, simpler methods provide inconsistent results, because they are dependent upon single-uptake dyes, which do not effectively discriminate between the various states of cellular demise. Therefore, there is a crucial need for analytical methods that provide rapid, robust and reproducible count and viability data to enable the efficient, daily execution of cellular research.
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