Muse™ Cell Analyzer Assays

See Cell Health Data Effortlessly

The Muse™ Cell Analyzer makes 3-parameter, quantitative cell analysis unbelievably simple. For the assays you rely on most, we've developed optimized kits, validated for robust performance on the Muse Cell Analyzer. Spend less time with experimental setup, avoid reagent waste and save money — we’ve done all the work for you.

Cell Health Assays Apoptosis Assays Cell Signalling Assays Immunology Assays

Muse Count & Viability Assay Kit

(Cat. No. MCH100102)

This Cell Analysis Assay enables rapid and reliable determinations of viability and total cell counts
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The Muse Count and Viability Kit provides rapid and reliable determinations of viability and total cell count using the Muse Cell Analyzer. Precise, accurate assessments can be made with a wide variety of cell types.

This simple no-wash, mix-and-read kit accurately determines absolute total cell counts and viability, based on differential permeabilities of two DNA-binding dyes. The nuclear dye stains only nucleated cells, while the viability dye brightly stains dying and dead cells. This proprietary combination of dyes enables the kit to distinguish viable and dead cells. Additionally, debris is excluded from results based on negative staining with the nuclear dye.

Muse Cell Cycle Assay Kit

(Cat. No. MCH100106)

Experience an easy Cell Cycle Protocol with this Cell Cycle Assay designed for the Muse Cell Analyzer
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The Muse Cell Cycle Kit can be used for measuring G0/G1, S, and G2/M phase distributions. The assay is based on the established method of whole-cell staining with propidium iodide (PI) and the single reagent contains everything you need for your assay already in solution.

Using this easy and convenient kit on the Muse Cell Analyzer will give you quick answers to your questions on cell cycle for your most important questions in cancer research, cell signaling, cell differentiation, and more.

Muse Annexin V & Dead Cell Assay Kit

(Cat. No. MCH100105)

This apoptosis assay follows an easy protocol to detect cells in various stage of apoptosis
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The Muse Annexin V & Dead Cell Kit offers a method to detect cells in various stages of apoptosis. The assay relies on the binding of fluorescently labeled Annexin V to phosphatidylserine (PS), which translocates to the outer surface of the cell membrane upon the onset of apoptosis.

The assay is a simple, mix-and-read assay that is highly sensitive and reproducible. The Muse Annexin V & Dead Cell Kit uses a single reagent and two stains to reliably stain and differentiate live, dead and apoptotic cells. Early in the apoptotic pathway, molecules of PS are translocated to the outer surface of the cell membrane. Annexin V-PE is used to to detect PS on the external membrane of apoptotic cells. 7-AAD, because it is excluded from live and healthy cells, permeates the late-stage apoptotic and dead cells. The Muse Cell Analyzer walks you step by step through gating up to 4 distinct cell populations: dead cells, live cells, early and late apoptotic cells.

Muse Caspase-3/7 Assay Kit

(Cat. No. MCH100108)

The Muse Caspase-3/7 Kit allows for the facile, rapid, and quantitative measurements of two important cell health parameters simultaneously; (1) apoptotic status based on Caspase-3.7 activation and (2) cellular plasma membrane permeabilization and cell death. The Muse Caspase-3/7 Kit simultaneously determines the count and percentage of cells in various stages of apoptosis based on activity of executioner caspases namely, Caspase-3/7 activity in combination with a dead cell dye. The kit utilizes (1) a novel Muse Caspase-3/7 reagent NucView for the detection of Caspase-3/7 activity and (2) a cell death dye that provides information on membrane integrity or cell death.

The assay provides relative percentage of cells that are live, in the early and late stage of apoptosis, and cell death on both adherent and suspension on the Muse Cell Analyzer. Minimal sample preparation is required in this no-wash, mix and read assay to obtain accurate and precise results.

Muse Mitopotential Assay Kit

(Cat. No. MCH100110)

Cell Health experiments are made easier with Muse Cell Analyzer and the Mitopotential assay kit
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The Muse MitoPotential Assay allows for the simultaneous measurement of 2 important cell health parameters; change in mitochondrial potential, considered an early hallmark of apoptosis, and cellular plasma membrane permeabilization or cell death. The Muse MitoPotential Assay utilizes the MitoPotential Reagent to detect changes the mitochondrial membrane potential. A dead cell marker is also used as an indicator of cell membrane structural integrity. It is excluded from live, healthy cells, as well as early apoptotic cells. Quantitative data (percentages and concentrations) is generated for 4 populations of cells: live, depolarized, depolarized/dead, and dead cells.

Minimal sample preparation is required in this no-wash, mix –and-read assay to obtain accurate and precise results.

Muse MultiCaspase Assay Kit

(Cat. No. MCH100109)

The Muse MultiCaspase Kit allows for the facile, rapid, and quantitative measurements of two important cell health parameters simultaneously—caspase activation and cellular plasma membrane permeabilization and cell death. Caspases (cysteinyl-directed aspartate-specific proteases) are cysteine proteases that play a central role in propagating the process of programmed cell death (apoptosis) in response to proapoptotic signals. In addition several caspases have also been found to have non-apoptotic roles in nonapoptotic functions of caspases in inflammation,mediating immunity, cell fate specification, cell survival, cell cycle regulation, cell proliferation, and cell migration. Muse MultiCaspase Assay is a Pan Caspase assay that can detect the presence of multiple caspases (caspase-1, 3, 4, 5, 6, 7, 8, and 9). The assay simultaneously determines the count and percentage of cells with caspase activity, in combination with a dead cell dye (7AAD). It provides relative percentages of cells that are either live, exhibiting caspase activity, and dead, for both adherent and suspension cultures on the Muse Cell Analyzer.

Minimal sample preparation is required in this no-wash, mix-and-read assay to obtain accurate and precise results. When samples are prepared with this reagent and acquired on the Muse Cell Analyzer, the software provides the following data:


The Multicaspase Assay kit determines the count and percentage of cells with caspase activity, in combination with a dead cell dye (7AAD)

Muse H2A.X Activation Dual Detection Assay Kit

(Cat. No. MCH200101)

Muse Activation Dual Detection kits include a pair of antibodies that bind to the same protein; one to detect total protein expression and another to detect the phosphorylated form of the same target. Using two parameter analysis, we can achieve target specific detection of phosphorylation and eliminate false positives while enhancing the signal to noise ratio. Data generated using the Muse Cell Analyzer with the Muse software provides:

The Muse H2A.X Activation Dual Detection Kit includes two directly conjugated antibodies, a phospho-specific anti-phospho-Histone H2A.X (Ser139)-Alexa Fluor®555 and an anti-Histone H2A.X-PECy5 conjugated antibody to measure total levels of Histone H2A.X. This two color kit is designed to detect the extent of Histone H2A.X pathway activation by measuring H2A.X phosphorylation relative to the total H2A.X expression in any given cell population, resulting in a normalized and accurate measurement of H2A.X activation after stimulation. Simultaneous measurement of both total and phospho-Histone H2A.X confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed cell population. Using this antibody pair provides a sensitive and valuable tool to study the factors that induce DNA damage and/or affect DNA repair, and allow one to explore the linkage between DNA damage, cell cycle checkpoints, and initiation of apoptosis.

Muse PI3K Activation Dual Detection Assay Kit

(Cat. No. MCH200103)

Muse Activation Dual Detection kits include a pair of antibodies that bind to the same protein; one to detect total protein expression and another to detect the phosphorylated form of the same target. Using two parameter analysis, we can achieve target specific detection of phosphorylation and eliminate false positives while enhancing the signal to noise ratio. Data generated using the Muse Cell Analyzer with the Muse software provides: The Muse PI3K Activation Dual Detection Kit includes two directly conjugated antibodies, a phospho-specific anti-phospho-Akt (Ser473), Alexa Fluor®555 and an anti-Akt, PECy5 conjugated antibody to measure total levels of Akt. This two color kit is designed to measure the extent of Akt phosphorylation relative to the total Akt expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of PI3K activation after stimulation. Moreover, simultaneous measurement of both total and phospho-Akt confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed population. Sufficient reagents are provided to perform 50 tests.

Muse MAPK Activation Dual Detection Assay Kit

(Cat. No. MCH200104)

Muse Activation Dual Detection kits include a pair of antibodies that bind to the same protein; one to detect total protein expression and another to detect the phosphorylated form of the same target. Using two parameter analysis, we can achieve target specific detection of phosphorylation and eliminate false positives while enhancing the signal to noise ratio. Data generated using the Muse Cell Analyzer with the Muse software provides:

The Muse MAPK Activation Dual Detection Kit includes two directly conjugated antibodies, a phospho-specific anti-phospho-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187)-Phycoerythrin and an anti-ERK1/2-PECy5 conjugated antibody to measure total levels of ERK. This two color kit is designed to measure the extent of MAPK phosphorylation relative to the total MAPK expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of MAPK activation after stimulation. Moreover, simultaneous measurement of both total and phospho-ERK1/2 confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed population. Sufficient reagents are provided to perform 50 tests.

Muse Bcl-2 Activation Dual Detection Assay Kit

(Cat. No. MCH200105)

Muse Activation Dual Detection kits include a pair of antibodies that bind to the same protein; one to detect total protein expression and another to detect the phosphorylated form of the same target. Using two parameter analysis, we can achieve target specific detection of phosphorylation and eliminate false positives while enhancing the signal to noise ratio. Data generated using the Muse Cell Analyzer with the Muse software provides:

The Muse Bcl-2 Activation Dual Detection Kit includes two directly conjugated antibodies, a phospho-specific anti-phospho-Bcl-2 (Ser70)-Alexa Fluor®555 and an anti-Bcl-2-PECy5 conjugated antibody to measure total levels of Bcl-2 expression. This two color kit is designed to detect the extent of Bcl-2 pathway activation by measuring Bcl-2 phosphorylation relative to the total Bcl-2 expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of Bcl-2 activation after treatment. Moreover, simultaneous measurement of both total and phospho-Bcl-2 confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed cell population. Sufficient reagents are provided to perform 50 tests.

Muse EGFR-RTK Activation Dual Detection Assay Kit

(Cat. No. MCH200102)

Muse Activation Dual Detection kits include a pair of antibodies that bind to the same protein; one to detect total protein expression and another to detect the phosphorylated form of the same target. Using two parameter analysis, we can achieve target specific detection of phosphorylation and eliminate false positives while enhancing the signal to noise ratio. Data generated using the Muse Cell Analyzer with the Muse software provides:

The Muse EGFR-RTK Activation Dual Detection Kit includes two directly conjugated antibodies, a phospho-specific anti-phospho-EGFR (Tyr1173)-Alexa Fluor®555 and an anti-EGFR-PECy5 conjugated antibody to measure total levels of EGFR expression. This two color kit is designed to detect the extent of EGFR pathway activation by measuring EGFR phosphorylation relative to the total EGFR expression in any given cell population, resulting in a normalized and accurate measurement of EGFR activation after stimulation. Simultaneous measurement of both total and phospho-EGFR confirms target specificity of the phosphorylation event, and provides an enhanced and more reliable detection of the phospho: total ratio within a mixed cell population. Sufficient reagents are provided to perform 50 tests.

Muse Human CD8 T Cell Kit

(Cat. No. MIM100102)

The Muse Human CD8 T Cell Kit is used in laboratory research studies to determine the CD8 T-cell count, total lymphocyte count, and CD 8 T-cell percent of lymphocytes in human whole blood and peripheral mononuclear cell (PBMC) samples. Identification and enumeration of cytotoxic CD8 T-cell populations are useful in understanding immune response and status, and in the research of disease mechanisms, such as autoimmune diseases, immunodeficiency.

The Muse Human CD8 T Cell Kit allows for the detection and identification of lymphocytes and CD8 T lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. The CD8 antibody allows the identification of CD8, a 68-kDa transmembrane glycoprotein expressed by class I major histocompatibility complex restricted, mature suppressor/cytotoxic T cells, the great majority of cortical thymocytes and approximately 30% of medullary thymocytes. In addition a proportion of γδ T cells and NK cells express CD8. Inclusion of several proprietary antibodies in the anti-lymphocyte cocktail allows for the unique identification of the CD8 cytotoxic T cells.

Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Muse Cell Analyzer provides:

The Muse Human CD8 T Cell Assay is for use with the Muse Cell Analyzer. The Muse System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps. Sufficient reagent is provided for the preparation and analysis of 100 tests.

CAUTION: This kit should not be used for patient diagnosis or patient management. For Research Use Only; not for use in diagnostic procedures.

Muse Human B Cell Kit

(Cat. No. MIM100103)

The Muse Human B Cell Kit is for use in laboratory research studies to determine the B cell count, total lymphocyte count, and B cell percent of lymphocytes in human whole blood and peripheral blood mononuclear cell (PBMC) samples. The characterization of B cell counts and percentage is important in multiple areas, such as understanding humoral immune response, research on mechanisms of autoimmune diseases, factors that influence T-cell responses, and for improved vaccine development.

The Muse Human B Cell Kit detects and identifies lymphocytes and CD19 B lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. CD19 is considered to be a characteristic B cell marker and therefore commonly used in the routine immunophenotyping of B cells. Monoclonal antibodies clustered as CD19 detect all peripheral blood B cells.4 The simplified identification can be of great utility in understanding the role of B lymphocytes in development, response, and mechanism of disease.

Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Muse Cell Analyzer provides:

The Muse Human B Cell Assay is for use with the Muse Cell Analyzer. The Muse System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps.

Sufficient reagent is provided for the preparation and analysis of 100 tests.

CAUTION: This kit should not be used for patient diagnosis or patient management. For Research Use Only; not for use in diagnostic procedures.

Muse Human CD4 T Cell Kit

(Cat. No. MIM100101)

The Muse Human CD4 T Cell Assay allows you to determine the concentration and percentages of CD4 T cells and lymphocytes in human whole blood or peripheral blood mononuclear cells (PBMCs). Characterizing CD4 T cell counts and percentages is important in evaluating immune status, understanding disease mechanism, in vaccine research and immunotoxicity studies. The Muse Human CD4 T Cell Kit allows for the detection and identification of lymphocytes and CD4 T lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. The CD4 antibody identifies human helper/inducer CD4+ T cell (HLA Class II reactive) and recognizes a 60,000 Da surface antigen. Monocytes also express CD4 but at a lower density, and have no co-expression of the other antibodies present in the anti-lymphocyte cocktail; hence can be distinguished from CD4 T Cells in this kit. The simplified identification can be of great utility in understanding the role of CD4 T lymphocytes in development, response, and mechanism of disease.

Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Muse Cell Analyzer provides:

The Muse Human CD4 T Cell Assay is for use with the Muse Cell Analyzer. The Muse System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps. Sufficient reagent is provided for the preparation and analysis of 100 tests.

CAUTION: This kit should not be used for patient diagnosis or patient management. For Research Use Only; not for use in diagnostic procedures.

Muse™Human Lymphocyte CD25 Kit

(Cat. No. MIM100105)

The Muse™Human Lymphocyte CD25 Kit is for use in laboratory research studies to determine the CD25 lymphocyte count, total lymphocyte count, and CD25 percent of total lymphocytes in human whole blood and PBMC samples. CD25 (IL-2 receptor á-chain) is an activation marker expressed on the surface of activated lymphocytes including T and B lymphocytes and NK cells. It is also expressed constitutively on the surface of small sub-populations of T cells. The study of CD25 expression on lymphocytes is important in understanding immune response and development, in immuno-modulatory mechanisms, and in the research of disease mechanisms. The Muse™ Human Lymphocyte CD25 Kit detects and identifies total lymphocytes and CD25 lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. The assay includes an antilymphocyte cocktail that identifies the total lymphocyte population and a CD25 antibody that identifies mid to late activation marker CD25..

Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Muse Cell Analyzer provides:

The Muse™ Human Lymphocyte CD25 Assay is for use with the Muse™ Cell Analyzer. The Muse™ System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse™ Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps.

Muse™ Human Lymphocyte CD69 Kit

(Cat. No. MIM100104)

The Muse™ Human Lymphocyte CD69 Kit is for use in laboratory research studies to determine the CD69(+) lymphocyte count, total lymphocyte count, and CD69 percent of total lymphocytes in human whole blood and peripheral blood mononuclear cell (PBMC) samples. CD69 is an early-activation marker expressed on the surface of activated lymphocytes including T and B lymphocytes and NK cells. The study of CD69 expression on lymphocytes is important in understanding immune response and development, in immuno-modulatory mechanisms and in the research of diseases. The Muse Human Lymphocyte CD69 Kit detects and identifies lymphocytes and CD69 lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. The Muse™ Human Lymphocyte CD69 Assay uses an antilymphocyte cocktail that identifies the total lymphocyte population and a CD69 antibody that binds to the CD69 early activation marker expressed on lymphocytes. The kit also includes an isotype control antibody.

Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. The software readout on the Muse Cell Analyzer provides:

The Muse™ Human Lymphocyte CD69 Assay is for use with the Muse™ Cell Analyzer. The Muse™ System makes sophisticated fluorescent-based analysis fast, easy, convenient, and affordable. Sample preparation is minimal, and after loading samples onto the Muse™ Cell Analyzer, the intuitive software provides detailed or summary analysis of your cell sample in a few short steps.