Muse™ Cell Analysis Principle

The Muse™ Cell Analyzer uses miniaturized fluorescence detection and microcapillary technology to deliver truly accurate, precise and quantitative cell analysis compared to other methods.

Sample analysis progresses from sample setup to analysis and results in 5 steps or fewer. The user interface is specifically tailored for streamlined applications, so you spend less time with experimental setup and analysis, and have more time for breakthrough research.

Laser-based fluorescence detection

The Muse Cell Analysis technology is based on Laser-based fluorescence detection to provide more quantitative results
Click to enlarge.
The system delivers high-performance cell analysis using a microcapillary and miniaturized optics which occupy one-tenth the space of a typical cytometer. Laser-based fluorescence detection of each cell event can evaluate up to 3 cellular parameters. As a result, Muse™ provides more quantitative results than image-based systems, which only examine up to 2 parameters, are time-consuming and ultimately provide less quantitative data.

A Basis for Sensitive Detection


Click to enlarge.
Fluorescence emitted by a cellular event is detected as a voltage pulse, described in diagram to the left. The width and height are proportional to the amount of fluorescence intensity for each cell event, and this leads to highly quantitative measurements of labeled cells.

Discover how Muse™ Cell Analyzer works, Step-by-Step Protocol

Sample Preparation Load Sample Adjust Settings Acquire Data Analyze Data

Muse Cell Analyzer Protocol - Step 1: Sample Preparation

Most Muse™ assays are mix-and-read. Simply bring your cells into suspension, add the reagent and acquire data on the Muse™ analyzer.

For more information on specific sample preparation for each assay click on the assay kit's name:

Cell Health Assays Apoptosis Assays Cell Signalling Assays Immunology Assays

Muse™ Cell Analyzer Protocol - Step 2: Load Sample


Click to enlarge.
Insert the sample into the loader arm and manually lift the sample into the instrument. A special safeguard ensures that uncapped tubes are loaded. Once the sampling process is complete, the automated loader allows the tube to drop.

Muse™ Cell Analyzer Protocol - Step 3: Adjust Settings

The Muse cell analyzer user interface enables you to quickly adjust your settings and select the population of interest
Click to enlarge.

Muse™ Cell Analyzer Protocol - Step 4: Acquire Data

While acquiring your data with Muse Cell Analyzer, you can monitor each step of the protocol through an easy-to-use interface
Click to enlarge.
After adjusting your settings just once, you are ready to aquire all your samples in succession. Enter your sample information, load your sample and touch “Run” to begin data acquisition.

Muse™ Cell Analyzer Protocol - Step 5: Analyze Data

Look at your cell analysis results the way you need by selecting among the different format as well as export this results for use on another device Look at your cell analysis results the way you need by selecting among the different format as well as export this results for use on another device
Look at your cell analysis results the way you need by selecting among the different format as well as export this results for use on another device
Click to enlarge.
 It just takes a few quick selections to obtain results! Data shown are customized for each assay. You can choose to view either the statistics only or your dotplots. If necessary, you can view dotplots to make modifications to gating parameters accordingly.

Results are displayed in a report that can be previewed and then printed or converted to PDF format. The raw data files and .csv format files can also be exported as well. Data sets that have been exported can be re-analyzed on the instrument later.