Enhance your cell Analysis data using Muse™ Cell Analyzer
Using Muse Cell Analyzer, discover and experience a new way to approach Cell Analysis experiments: more accurate, more precise and more reliable .
More Accurate Cell Analysis
Click to enlarge.
The Muse™ Cell Analyzer counts cells more accurately than manual hemocytometry or image-based automated analysis. Multiple adherent and suspension cell types were harvested, diluted and counted. Cell counts from all three methods were averaged to obtain “theoretical cell concentration”. Each point represents average cell concentration of 3 replicates, and each data series was fit with linear regression. Muse™ cell analysis data were correlated with theoretical concentration with slope closest to 1, indicating superior accuracy.
More Precise Cell Analysis
The Muse™ Cell Analyzer counts cells and measures viability more precisely with smaller coefficients of variation (%CV) than manual hemocytometry or image-based automated analysis. While image-based automated counting methods and manual hemocytometry displayed broader ranges of %CVs, the Muse™ Cell Analyzer exhibited a narrow range of %CVs and consistently provided %CVs less than 10% over the entire range of samples tested. Higher %CVs were observed for the Trypan blue-based methods, particularly at lower cell concentrations.
Analysis
Cell Concentration
Viability
Average % CV
% CV Range
Average % CV
% CV Range
Image-based Automated Counter
9.2%
1.2-23.3%
3.7%
0.8-12.1%
Manual Hemocytometer
6.3%
0.5-15.3%
4.5%
0.5-9.2%
Muse™
4.0%
0.3-8.8%
2.2%
0.4-5.6%
The Muse™ Cell Analyzer provides superior precision for cell concentration and viability measurements, compared to Trypan blue-based analyses. Data are based on triplicate measurements of 30 cellular samples from suspension and adherent cell lines at multiple concentrations and viabilities.
More Reliable Cell Analysis
Click to enlarge.
The Muse™ Cell Analyzer performs with high linearity across multiple cell lines and a wide sample concentration range. The concentration results for serial dilutions of 5 representative cell lines are shown, which include both adherent and suspension cells. Each point represents the average of three samplings. Expected cell concentrations were calculated by measuring the stock sample concentration with the Muse™ Cell Analyzer, then dividing by the dilution factor to obtain theoretical concentrations of diluted samples. Data show the comparison of observed vs. expected cell concentrations.
;)
;)