Multiplex Assays - Millipore

SignalProfiler™ Pathways (multiplex assays)

Akt - 2 Plex
Akt/mTOR Phospho - 11 Plex
Akt/mTOR Total - 11 Plex
Angiogenesis RTK Phospho - 10 Plex
Angiogenesis RTK Total - 10 Plex
Early Apoptosis - 7 Plex
EGFR Profiler - 8 Plex
Erk / MAPK 1/2 - 2 Plex
Heat Shock Protein - 5 Plex
Late Apoptosis - 3 plex
MAPK/SAPK - 10 plex
Mitogenesis RTK Phospho - 7 Plex
Mitogenesis RTK Total - 7 Plex
Multi-Pathway - 9 Plex
Src Family Kinase - 7 Plex
STAT - 5 Plex
T Cell Receptor - 7 plex
TGFβ - 6 Plex

Detailed description of pathways

Human STAT - 5 Plex Catalogue Number: 48-610 (nonmagnetic) Catalogue Number: 48-610MAG (magnetic) STAT1 (Tyr701) STAT2 (Tyr690) STAT3 (Tyr705) STAT5A/B (Tyr694/699) STAT6 (Tyr641)	Human Multi-Pathway 9 Plex Catalogue Number: 48-680MAG (magnetic) Akt (Ser473) CREB (Ser133) Erk/MAPK 1/2 (Thr185/Tyr187) NFκB (Ser536) JNK/SAPK1 (Thr183/Tyr185) p38/SAPK2A/B (Thr180/Tyr182) p70 S6 Kinase (Thr412) STAT3 (Ser727) STAT5A/B (Tyr694/699)

Human T Cell Receptor – 7 Plex Catalogue Number: 48-690 (magnetic) CD3ε (pan Tyr) CREB (Ser133) Erk/MAPK 1/2 (Thr185/Tyr187) LAT (pan Tyr) Lck (pan Tyr) Syk (pan Tyr) ZAP-70 (pan Tyr)	Human MAP/SAPK 10 Plex Catalogue Number: 48-660 (magnetic) ATF2 (Thr69/71) c-Jun (Ser73) Erk/MAPK 1/2 (Thr185/Tyr187) HSP27 (Ser78) JNK/SAPK1 (Thr183/Tyr185) MEK1 (Ser222) MSK1 (Ser212) p38/SAPK2A/B (Thr180/Tyr182) p53 (Ser15) STAT1 (Tyr701)

Src Family Kinase Active Site 8 Plex Catalogue Number: 48-650MAG (magnetic) Blk (Tyr389) Fgr (Tyr412) Fyn (Tyr420) Hck (Tyr411) Lck (Tyr394) Src (Tyr419) Yes (Tyr421)	Human Late Apoptosis 3 Plex Catalogue Number: 48-670MAG (magnetic) Caspase 3 (Active) GAPDH (Total) PARP (Cleaved)

	MILLIPLEX MAP Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit Catalogue Number: 48-611MAG (magnetic) Akt (Ser473) GSK3α (Ser21) GSK3β (Ser9) IGF1R (Tyr1135/Tyr1136) IR (Tyr1162/Tyr1163) IRS1 (Ser312) PTEN (Ser380) RPS6 (Ser235/Ser236) TSC2 (Ser939) mTOR (Ser2448) p70S6K (Thr412)

MILLIPLEX MAP Total Akt/mTOR Magnetic Bead 11-Plex Kit Catalogue Number: 48-612MAG (magnetic) Akt (Total) GSK3α (Total) GSK3β (Total) IGF-1R (Total) IR (Total) IRS1 (Total) PTEN (Total) RPS6 (Total) TSC2 (Total) mTOR (Total) p70S6K (Total)	MILLIPLEX MAP EGFR Profiler Magnetic Bead 8-Plex Kit Catalogue Number: 48-613MAG (magnetic) EGFR (Ser1047) EGFR (Thr654) EGFR (Thr669) EGFR (Tyr1045) EGFR (Tyr1068) EGFR (Tyr1086) EGFR (Tyr845) EGFR (Total)

MILLIPLEX MAP Heat Shock Protein Magnetic Bead Panel Catalogue Number: 48-615MAG (magnetic) HSP27 (Total) HSP27 (pS78/pS82) HSP60 HSP70 HSP90α	MILLIPLEX MAP Human Phospho Mitogenesis RTK Magnetic Bead 7-Plex Kit Catalogue Number: 48-672MAG (magnetic) c-Met/HGFR (panTyr) EGFR (pan Tyr) ErbB2/HER2 (pan Tyr) ErbB3/HER3 (panTyr) ErbB4/HER4 (panTyr) IGF-1R (pan Tyr) IR (panTyr)

MILLIPLEX MAP Human Total Mitogenesis RTK Magnetic Bead 7-Plex Kit Catalogue Number: 48-671MAG (magnetic) c-Met/HGFR (total) EGFR (total) ErbB2/HER2 (total) ErbB3/HER3 (total) ErbB4/HER4 (total) IGF-1R (total) IR (total)	MILLIPLEX MAP Human TGFβ Singaling Pathway Magnetic Bead 6-Plex Kit Catalogue Number: 48-614MAG (magnetic) Akt (Ser473) ERK (Thr185/Tyr187) Smad2 (Ser465/Ser467) Smad3 (Ser423/Ser425) Smad4 (total) TGFβRII (total)

Src Family Kinase Active Site — 8 Plex Catalogue Number: 48-650MAG (magnetic) Blk (Tyr389) Fgr (Tyr412) Fyn (Tyr420) Hck (Tyr411) Lck (Tyr394) Src (Tyr419) Yes (Tyr421)	Human Angiogenesis RTK — 10 Plex Catalogue Number: 48-674MAG (magnetic) c-Kit 3 (pan Tyr) Flt3 3 (pan Tyr) M-CSFR 3 (pan Tyr) PDGFRα 3 (pan Tyr) PDGFRβ 3 (pan Tyr) Tie1 3 (pan Tyr) Tie2 3 (pan Tyr) VEGF R1 3 (pan Tyr) VEGF R2 3 (pan Tyr) VEGF R3 3 (pan Tyr)

Human Total Angiogenesis RTK — 10 Plex Catalogue Number: 48-673MAG (magnetic) c-Kit Flt3 M-CSFR PDGFRα PDGFRβ Tie1 Tie2 VEGF R1 VEGF R2 VEGF R3	Early Apoptosis — 7 Plex Catalogue Number: 48-669MAG (magnetic) Analyte Total Phosphorylated Akt 3 (Ser473) BAD 3 (Ser112) Bcl-2 3 (Ser70) Active Caspase 8 3 Active Caspase 9 3 JNK 3 (Thr183/Tyr185) p53 3 (Ser46)

Akt — 2 Plex Catalogue Number: 48-618MAG (magnetic) Analyte Total Phosphorylated Akt 3 Akt 3 (Ser473)	Erk / MAPK 1/2 — 2 Plex Catalogue Number: 48-619MAG (magnetic) Analyte Total Phosphorylated Erk 3 Erk 3 (Thr185/Tyr187)