Chemiluminescent Detection, HRP Substrates, Luminata

Chemiluminescent Detection

Chemiluminescent detection uses an enzyme to catalyze a reaction that results in the production of visible light. Detection is achieved by either exposing the blot to X-ray film or acquiring the image directly in a chemiluminescence- compatible digital imaging system, usually equipped with highly-cooled CCD cameras to avoid electronic noise. Reprobing is possible with chemiluminescent substrates.

There are a variety of chemiluminescent substrates offering researchers different levels of sensitivity of detection. Traditional or low-sensitivity substrates allow protein detection at the picogram level. While these substrates may be appropriate for routine applications, they cannot detect low abundance proteins. New, high-sensitivity substrates, such as Immobilon substrates, allow visualization of proteins at the femtogram level. However, use of these powerful substrates often requires optimization of primary and secondary antibody concentrations.

Luminata^™ Western HRP Substrates

Compare Luminata Chemiluminescent Detection Substrates

Chemiluminescent HRP (horseradish peroxidase) substrates (also known as ECL reagents) are the most sensitive reagents used in the detection of Western blots.

The Luminata^™ Western HRP Substrates are a family of three premixed HRP substrates, which offer several advantages over other detection reagents. Pour directly onto the Western membrane without worrying about pipetting error. The Luminata™ Classico, Crescendo and Forte substrates cover a broad range of sensitivities.

Advantages of Luminata Chemiluminescent Detection Substrates

Premixed for convenience and to avoid cross contamination of the reagents.
Broad range of sensitivities to satisfy all detection needs.
Consistent results with less pipetting error.
Stable at 4 °C or room temperature so you can store it on your own bench.
Best detection performance relative to competition.
Compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.

SignalBoost^™ Immunoreaction Enhancer Kit

SignalBoost Chemiluminescent Detection Immunoreaction Enhancer Kit

Detecting proteins in a Western can be difficult for multiple reasons (low protein abundance, low affinity antibody, epitope availability, etc.). SignalBoost^™ mmunoreaction Enhancer can amplify your signals so you can get your data more quickly and spend less time troubleshooting.

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ReBlot^™ Antibody Stripping Solution

ReBlot Western Blotting Antibody Stripping Solution

This quick stripping reagent is the product of choice for regenerating Western blots. ReBlot^™ Plus reagents efficiently strip probed blots of bound antibodies. ReBlot^™ Plus reagents are available in two formulations, "Mild" and "Strong".

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