Immobilon-PSQ Membrane, Transfer Membrane

Immobilon^®-P^SQ Transfer Membrane

Featured Benefit
Immobilon^®-PSQ membrane prevents blow-through. A sheet of Immobilon^®-PSQ was placed behind a strip of Immobilon^®-P and Immobilon^®-PSQ to capture proteins that passed through. After the transfer, The backup Immobilon^®-PSQ membrane showed that no proteins passed through the Immobilon^®-PSQ membrane (lanes 7 and 8).

This membrane has higher protein adsorption and sequencing yields than other membranes.

0.2 µm pore size and a large internal structure
Higher protein adsorption and sequencing yields than other membranes
Recommended for blotting proteins <20 kDa
Prevents blow-through of low molecular weight proteins

Immobilon^®-P^SQ Membrane Prevents
Blow-through

Molecular weight standards (lanes 1, 3, 5, 7) and calf liver lysate (lanes 2, 4, 6, 8) were transferred to Immobilon^®-P or Immobilon^®-P^SQ membranes. A sheet of Immobilon^®-P^SQ transfer membrane was placed behind the primary membranes to capture proteins that passed through (lanes 5 and 6 behind Immobilon^®-P; lanes 7 and 8 behind Immobilon^®-P^SQ membrane).

Immobilon®-PSQ Transfer Membrane

Immobilon®-PSQ Membrane PreventsBlow-through

Immobilon^®-P^SQ Transfer Membrane

Immobilon^®-P^SQ Membrane Prevents
Blow-through