Lead Discovery - IC50Profiler
Rapidly estimate inhibitor potency against specific targets. The IC50Profiler service returns IC50 curves against any kinase on our KinaseProfiler panel.Millipore's IC50Profiler service gives you:
- "Gold Standard" radiometric format
- 10-point curves, duplicated assay points, and quality controlled data
- Choice of 10µM or Km ATP
- Customer-specified top compound concentration
- Results in two-weeks
The IC50Profiler service provides for the rapid generation of IC50 curves on customer-supplied compounds against any of the kinases in our KinaseProfiler panel.
The IC50 value is estimated by preparing a 10 point curve using a ½ log dilution series (for example, a typical curve may be prepared using the following compound concentrations; 100 µM, 30 µM, 10 µM, 3 µM, 1 µM, 0.3 µM, 0.1 µM, 0.03 µM, 0.01 µM and 0 µM).
Millipore guarantees a two-week turnaround on IC50Profiler. All processes and procedures are performed according to strict QC guidelines and confidentiality is guaranteed.
| Service | Screening Limit/ Turnaround |
Description |
| KinaseProfiler Service | NOW ONLY 2-weeks! | All Kinases in the Panel ! |
| IC50Profiler | 2 weeks | For rapid IC50 curve generation against any KinaseProfiler targets, select IC50Profiler |
EGFR activity was measured via incorporation of radioactivity from 10 µM γ -33P - ATP into polyGlu:Tyr in the presence of compound concentrations indicated.
Abl activity was measured via incorporation of radioactivity from 10 µM γ -33P - ATP into EAIYAAPFAKKK in the presence of compound concentrations indicated.
EGFR activity was measured via incorporation of radioactivity from 10 µM γ -33P - ATP into polyGlu:Tyr in the presence of compound concentrations indicated.
