Easy, Quantitative Detection of Protein Oxidation
Oxidative stress is a cellular condition particularly relevant to aging and age-related diseases, such as Alzheimer’s disease, Parkinson’s disease, and dementia. Millipore offers a complete line of qualitative and quantitative tools to help you study oxidative stress. Regardless of your throughput needs and preferred detection method, you’ll find a kit to improve your research.Fig. 1: Upstream & downstream applications of oxidative stress antibodies, OxyBLOT & OxyELISA.
NEW! The OxyELISA™ Oxidized Protein Quantitation Kit provides a sensitive methodology for detection and quantitation of proteins modified by oxygen free radicals and other reactive species. Oxygen-derived free radicals have been implicated in numerous physiological functions such as apoptosis, cancer, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases. As result of oxidative stress, oxygen free radicals and other reactive species introduce carbonyl groups into proteins in the cell. The OxyELISA kit detects these carbonyl groups, which are hallmarks of the cellular oxidative stress level. The kit uses a direct labeled monoclonal antibody that is readily detected without a secondary antibody, making the ELISA assay more specific, easier to use, and faster.
Fig. 2: Protein carbonylation of HeLA cells in response to increasing H2O2 treatment detected by OxyELISA Kit.
The OxyBlot™ Protein Oxidation Detection Kit provides the chemical and immunological reagents necessary to perform the immunoblot detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.
Ordering Information
Kits for Oxidative Stress Research
| Product Description | Qty/Pk | Catalogue No. |
| OxyELISA Oxidized Protein Quantitation Kit | 1 kit | S7250 |
| OxyBlot Oxidized Protein Detection Kit | 200 assays | S7150 |
| OxyBlot Protein Standard | 150 µL | S7151 |
| Nitrotyrosine ELISA Kit | 1 kit | 17-376 |
| Coming Soon: OxyICC for detection of protein oxidation in cell culture. | ||
Related Antibodies for Oxidative Stress Research
| Product Description | Qty/Pk | Catalogue No. |
| Anti-Nitrotyrosine | 100 µg | 06-284 |
| Anti-8-Hydroxydeoxyguanosine | 200 µL | AB5830 |
| Anti-8-Oxoguanine, clone 483.15 | 100 µL | MAB3560 |
| Anti-Superoxide Dismutase 1 | 50 µL | AB5482 |
| Anti-IDO (Indoleamine 2,3-Dioxygenase), clone 10.1 | 100 µg | 05-840 |
| Anti-Thioredoxin Reductase 1 (TrxR1) | 100 µg | 07-613 |
| Anti-Neuroketal | 200 µL | AB5611 |
| Anti-RAGE | 200 µL | AB5484 |
| Anti-MsrA | 200 µL | 07-338 |
| Anti-Hypoxia Inducible Factor 1 α, clone H1α67 | 100 µg | MAB5382 |
| Anti-nNOS/NOS I | 200 µL | 07-571 |
| Anti-iNOS/NOS II, NT | 200 µg | 06-573 |
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