Immunoprecipitate the Easy Way

With Millipore’s Catch and Release Kit!

Millipore’s Catch and Release® kit was designed to meet your needs by making IP faster, simpler, and more reproducible.

With Catch and Release, you can purify both native and denatured proteins using a variety of antibodies and sample types. The kits are even validated for Co-IP and IP-kinase assays (see references below).

Catch and Release versus Traditional IP

Greatest recovery in first elution

Elute either native or denatured protein

Description	Capacity	Cat. No.
Catch and Release Sample Kit	5 assays	17-500A
Catch and Release Kit	50 assays	17-500
Catch and Release High Throughput (HT) Kit – 96 well format	96 assays	17-501
Catch and Release Phosphotyrosine, clone 4G10 Sample Kit	5 assays	17-502A
Catch and Release Phosphotyrosine, clone 4G10 Kit	50 assays	17-502
Additional Products
Ultrafree-MC Centrifugal Filter Unit	25 units	UFC30HV25
Catch and Release Wash Buffer, 10X	15 mL	20-210
Catch and Release Denaturing Elution Buffer, 1X	4 mL	20-284
Catch and Release Non-Denaturing Elution Buffer, 4X	10 mL	20-209

Application references:

Maneechotesuwan, K. et al. (2007). Regulation of Th2 Cytokine Genes by p38 MAPK-Mediated Phosphorylation of GATA-3. J. Immunol. 178:2491-2498.

Nduati, V. et al. (2007). Leptin transcriptionally enhances peptide transporter (hPepT1) expression adn activity via the cAMP-response element-binding protein and Cdx2 transcription factors. J. Biol. Chem. 282:1359-1373.

Koncarevic, A. et al. (2007). The ubiquitin-protein ligase Nedd4 targets Notch1 in skeletal muscle and distinguishes the subset of atrophies caused by reduced muscle tension. FASEB J. 21:427-437.

Jin, C. & Felsenfeld, G. (2007). Nucleosome stability mediated by histone variants H3.3 and H2A.Z. Genes & Dev. 21:1519-1529.

Ohba, S. et al. (2007). Identification of a potent combination of osteogenic genes for bone regeneration using embryonic stem (ES) cell-based sensor. FASEB J. 21:1777-1787.

Tong, c. et al., (2007). C-Jun NH2-terminal kinase 1 plays a critical role in intestinal homeostasis and tumor suppression. Am. J. Phathol. 171:297-303.

Mateus, A. R. et al., (2007). EGFR regulates rhoA-GTP dependent cell motility in E-cadherin mutant cells. Hum. Mol. Genet. 16:1639-1647.

Wiese, S. et al., (2007). Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrKB. PNAS 104:17210-17215.

Kanayma, M. et al., (2007). Hepatocyte growth factor promotes colonic epithelial regeneration via Akt signaling. Am. J. Physiol. Gastrointest. Liver Physiol. 293:GG230-G239.

Judge, A. R. et al. (2007). Role for IkBalpha, but not c-Rel, in skeletal muscle atrophy. Am. J. Physiol. Cell Physiol. 292:C372-C382.

Qu, Z. et al. (2007). Bispecivic anti-CD20/22 antibodies inhibit B-cell lymphoma proliferation by a unique mechanism of action. Blood Epub. 10.1182/blood-2007-08-110072.

Edderkaoui, M. et al. (2007). Insulin-like growth factor-I receptor mediates the prosurvival effect of fibronectin. J. Biol. Chem. 282:26646-26655.

McGugan Jr., G. C. et al. (2007). Identification and biochemical characterization of unique secretory nucleases of the human enteric pathogen Entamoeba histolytica. J. Biol. Chem. 282:31789-31802.

Oguri, T. et al. (2006). Vimentin-Ser82 as a memory phosphorylation in astrocytes. Genes Cells 11:531-540.

Le, J. H. et al. (2006). Phospholipase D2 acts as an essential adaptor protein in the activation of Syk in antigen-stimulated mast cells. Blood 108:956-964.

Hongwei, Li, et al. (2006). The Histone methylatransferase SETDB1 and the DNA Methyltransferase DNMT3A interact directly and localize to promotors silenced in cancer cells. J. Biol. Chem. 281:19489-19500.

Guo, Can, et al (2006). The expression of p63 is associated with the differential stage in nasopharyngeal carcinoma and EBV infection. Journal of translational medicine [electronic resource] 4: 23. Pubmed Abstract (Applications: Co-IP)

Li, W., et al (2005). Srcasm modulates EGF and Src-kinase signaling in keratinocytes. J Biol Chem 280: 6036-46. Pubmed Abstract (Applications: Immunoprecipitation)

Sastri, M., et al (2005). A-kinase-interacting protein localizes protein kinase A in the nucleus. Proc Natl Acad Sci U S A 102: 349-54. Pubmed Abstract (Applications: Co-IP)

Choi, W. S., et al (2004). Activation of RBL-2H3 mast cells is dependent on tyrosine phosphorylation of phospholipase D2 by Fyn and Fgr. Mol Cell Biol 24: 6980-92. Pubmed Abstract (Applications: Immunoprecipitation, Kinase Assay)

Digicaylioglu, M., et al (2004). Acute neuroprotective synergy of erythropoietin and insulin-like growth factor I. Proc Natl Acad Sci U S A 101: 9855-60. Pubmed Abstract (Applications: Co-IP)

Lin, R. J., et al (2004). Blocking of the alpha interferon-induced Jak-Stat signaling pathway by Japanese encephalitis virus infection. J Virol 78: 9285-94. Pubmed Abstract (Applications: Immunoprecipitation)

Song, L. N., et al (2004). Antiandrogen effects of mifepristone on coactivator and corepressor interactions with the androgen receptor. Mol Endocrinol 18: 70-85. Pubmed Abstract (Applications: Co-IP)

Zhang, N., et al (2004). Elevated vascular endothelial growth factor production in islets improves islet graft vascularization. Diabetes 53: 963-70. Pubmed Abstract (Applications: Immunoprecipitation)

Altomonte, J., et al (2003). Inhibition of Foxo1 function is associated with improved fasting glycemia in diabetic mice. Am J Physiol Endocrinol Metab 285: E718-28. Pubmed Abstract (Applications: Immunoprecipitation)

He, W., et al (2003). Direct activation of cyclin-dependent kinase 2 by human papillomavirus E7. J Virol 77: 10566-74. Pubmed Abstract (Applications: Immunoprecipitation)

Jiang, X., et al (2003). Neonatal hypoxia-ischemia differentially upregulates MAGUKs and associated proteins in PSD-93-deficient mouse brain. Stroke 34: 2958-63. Pubmed Abstract (Applications: Co-I

Lennon, N. J., et al (2003). Dysferlin interacts with annexins A1 and A2 and mediates sarcolemmal wound-healing. J Biol Chem 278: 50466-73. Pubmed Abstract (Applications: Co-IP)

Petrie, K., et al (2003). The histone deacetylase 9 gene encodes multiple protein isoforms. J Biol Chem 278: 16059-72. Pubmed Abstract (Applications: Co-IP)

Qiao, L., et al (2003). Bile acid regulation of C/EBPbeta, CREB, and c-Jun function, via the extracellular signal-regulated kinase and c-Jun NH2-terminal kinase pathways, modulates the apoptotic response of hepatocytes. Mol Cell Biol 23: 3052-66. Pubmed Abstract (Applications: p38 Kinase Assay)

Brymora, A., et al (2001). Enhanced protein recovery and reproducibility from pull-down assays and immunoprecipitations using spin columns. Anal Biochem 295: 119-22. Pubmed Abstract (Applications: Spin Column Rapid Procedure)

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