Millipore - MilliTrace™ Constitutive GFP Reporter Neural Stem Cell Lines - New cell lines allow robust, in vivo monitoring of stem cell behavior

MilliTrace™ Constitutive GFP Reporter Neural Stem Cell Lines

New cell lines allow robust, in vivo monitoring of stem cell behavior
Billerica, Massachusetts—June 30, 2008—Millipore Corporation, a Life Science leader providing technologies, tools and services for bioscience research and biopharmaceutical manufacturing, today announced the availability of their MilliTrace primary rodent neural stem cell (NSC) lines that express green fluorescent protein (GFP) constitutively. GFP expression in these stem cells is the best way for researchers to monitor the behavior of specific populations of cells as they proliferate, migrate, and differentiate into various cell lineages, depending on developmental context. The MilliTrace cell lines are the first commercially available, GFP-expressing, karyotypically normal stem cell lines, and are supplied with optimized expansion medium.

Many immortal cell lines display unstable or aneuploid chromosomes; however, for stem cell lines to yield physiologically relevant results, it is important that the cells display normal chromosomes. Unlike human NSC cultures, rodent NSCs undergo prolonged self-renewal in culture without losing chromosomal stability. Researchers at Millipore have been able to isolate and propagate monolayer cultures of NSCs from two different regions of the rodent brain: the adult rat hippocampus and the embryonic mouse cortex. Even after introduction of constitutively expressing GFP, the NSCs from these tissues show normal karyotype, stability over more than 10 passages, and multipotency. Both cell lines can be readily differentiated into neurons, astrocytes, or oligodendrocytes, using appropriate cues.

Validated for high levels of GFP expression, stem cell marker expression, and multipotency, MilliTrace GFP Reporter Neural Stem Cell Lines can improve reproducibility and data quality for a variety of applications. Researchers can use MilliTrace cell lines to study cell-cell interactions in co-culture studies or contribution of NSCs to the stem cell niche in vivo. MilliTrace cell lines also enable high-throughput screens to discover agents that affect stem cell maintenance and differentiation.

Millipore has evolved from a high performance filtration products and services company into a leading provider of tools and services and a key partner of choice for Life Science customers. We’ve transformed ourselves through R&D investment, organic growth, and acquisitions such as Chemicon, Upstate, Linco, Celliance, Newport Bio Systems, NovAseptic AB and MicroSafe. The integrated Millipore offers more innovative technologies and stronger application support to streamline processes and provide consistently reliable results. The expert scientists in our Life Sciences business unit understand the complexity of Life Science research and can support our customers in their most difficult challenges in cell biology, stem cells, protein research, and cell signaling.

About Millipore

Millipore (NYSE: MIL) is a Life Science leader providing cutting-edge technologies, tools, and services for bioscience research and biopharmaceutical manufacturing. As a strategic partner, we collaborate with customers to confront the world’s challenging human health issues. From research to development to production, our scientific expertise and innovative solutions help customers tackle their most complex problems and achieve their goals. Millipore Corporation is an S&P 500 company with 6,000 employees in 47 countries worldwide. For more information, please contact Millipore Tech Service at 1-800-548-7853 or 951-676-8080 or visit www.millipore.com.

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