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Cell Culture Media Preparation

Millipore recommends running small-scale trials for capacity and throughput screening. This table provides details on process development devices for recommended filters and alternatives since the ideal solution will vary based on each fluid stream. The following order table is for recommended devices only.

Bacterial and Mycoplasma
Filter FormatArea/FilterCatalogue No.
OptiScale Capsules
    Single-layer Millipore Express SHR 0.1 um membrane
13.8 cm2SVEPA47FF3
    Double-layer Millipore Express SHR 0.1 um + prefilter layer
13.8 cm2SHVEA47FF3
    Durapore 0.1 um membrane
13.8 cm2SWLA47FF3
Triple-layer Polysep II Grade W1 media13.8 cm2SGW1A47FF3
Millistak+ Mini Capsule
Triple-layer Grade A1HC23 cm2MA1HC23HH3
    Single-layer Grade 75DE
23 cm2M75DE23HH3
Bacterial
Filter FormatArea/FilterCatalogue No.
OptiScale Capsules
    Double-layer Millipore Express SHC0.5 um + 0.2 um membrane
23 cm2SHGEA47FF3
    Durapore 0.22 um membrane
23 cm2SVGLA47FF3
Double-layer Polysep II Grade W3 media23 cm2SGW3A47FF3
Millistak+ Mini Capsules
Triple-layer Grade A1HC23 cm2MA1HC23HH3
    Single-layer Grade 75DE
23 cm2M75DE23HH3

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